高乌头不同溶剂萃取部分对小鼠的急性毒性研究

摘要

目的 比较高乌头不同溶剂萃取部分的急性毒性大小,筛选高乌头的毒性部位,为阐明高乌头的毒性成分提供依据.方法 采用系统溶剂法对高乌头95%乙醇提取物进行萃取,依次得到石油醚、三氯甲烷、乙酸乙酯、正丁醇、乙醇和水提6个不同的溶剂萃取部分,通过半数致死量(LD50)和最大给药量法对高乌头不同溶剂萃取部分的急性毒性进行比较研究.结果 高乌头三氯甲烷、水提及正丁醇部分LD50分别为89.65、1805.40、24409.41 mg/kg,95%可信区间分别为76.39~108.41、1521.60~2240.00、20422.54~24246.95;高乌头石油醚、乙酸乙酯及乙醇部分最大给药量分别为2686.01、3108.13、28376.21 mg/kg.结论 高乌头三氯甲烷部分毒性最大,乙醇部分毒性最小.%Objective To compare the toxicity of different extraction parts of Aconitum sinomontanum Nakai;To screen out"toxic sections"of Aconitum sinomontanum Nakai; To provide references for further study on toxicity components of Aconitum sinomontanum Nakai. Methods Systematic solvent method was used to extract the 95% ethanol extracts of Aconitum sinomontanum Nakai,and six different extraction fractions(petroleum ether,chloroform, ethyl acetate, butanol, alcohol and water) were obtained. Median lethal dose (LD50) and maximum dose method were used to conduct comparative study on acute toxicity of different extraction parts of Aconitum sinomontanum Nakai. Results Chloroform, water and butanol extractions in LD50of Aconitum sinomontanum Nakai were 89.65, 1805.40 and 24 409.41 mg/kg, and 95% confidence limits were 76.39~108.41, 1521.60~2240.00 and 20 422.54~24 246.95, respectively. The maximum dose of petroleum ether, ethyl acetate and alcohol extractions were 2686.01, 3108.13 and 28 376.21 mg/kg, respectively. Conclusion The maximum toxicity is the extracted section of chloroform, and the minimal toxicity is the extracted section of ethanol.