目的 观察健脾活血方对肝癌裸鼠皮下移植瘤模型细胞凋亡和血管生成的影响,探讨其可能的作用机制.方法 建立SMMC-7721肝癌荷瘤裸鼠模型.将60只雄性荷瘤裸鼠随机分为空白组、模型组、5-Fu组和健脾活血方高、中、低剂量组,每组10只.健脾活血方各剂量组给予相应浓度健脾活血方药液灌胃,每日1次,5-Fu组给予5-Fu隔日腹腔注射,连续2周.2周后比较各组裸鼠体质量、瘤质量和抑瘤率,免疫组化检测血管内皮生长因子(VEGF)和VEGF受体2(VEGFR-2)的蛋白表达,计算微血管密度(MVD),qRT-PCR检测Caspase-3和生存素(Survivin)的mRNA表达.结果 与模型组比较,各给药组VEGF、VEGFR-2蛋白表达,MVD和Caspase-3、Survivin mRNA表达均降低,其中5-Fu组和健脾活血方高剂量组作用最明显(P<0.05).结论 健脾活血方可能通过抑制血管生成和诱导细胞凋亡作用抑制肿瘤生长.%Objective To observe the effects of Jianpi Huoxue Prescription on the subcutaneous transplantation of tumor cell apoptosis and angiogenesis; To discuss its possible mechanism of action. Methods SMMC-7721 hepatoma mice models were established. 60 tumor bearing male mice were randomly divided into blank group, model group, 5-Fu group, Jianpi Huoxue Prescription high-, medium- and low-dosage groups, with 10 rats in each group. Model group and Jianpi Huoxue Prescription high-, medium- and low-dosage groups were given medicine with relevant concentrations for gavage, once a day. 5-Fu group was given 5-Fu for intraperitoneal injection every other day. The weight, tumor weight and tumor inhibitory rate of each group were compared two weeks later. The protein expressions of VEGF and VEGFR-2 were detected by immunohistochemistry, microvessel density (MVD) were calculated, and the expressions of Survivin and Caspase-3 mRNA were detected by RT-qPCR. Results Compared with model group, protein expressions of VEGF and VEGFR-2, MVD, Caspase-3 and Survivin mRNA decreased in Jianpi Huoxue Prescription high-, medium- and low-dosage groups, and the most obvious differences were in 5-Fu group and Jianpi Huoxue Prescription high-dosage group (P<0.05). Conclusion Jianpi Huoxue Prescription can inhibit tumor growth by inhibiting angiogenesis and inducing apoptosis.
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