新疆维吾尔族、汉族抗结核药物性肝损伤患者NO及NOS表达水平的研究

摘要

目的 探讨汉族、维吾尔族人群抗结核药物性肝损伤(ATDLI)患者血清 NO 及 NOS 的表达水平.方法 选取新疆维吾尔自治区胸科医院、石河子大学医学院第一附属医院2015年1月—2016年5月确诊的ATDLI患者,按民族分为汉族组和维吾尔族组.通过检测ATDLI患者血清中NO及NOS的表达水平,比较不同性别、身体质量指数(BMI)、肝功能损伤程度ATDLI患者的NO及NOS表达水平.结果 共收集ATDLI汉族组患者100例,维吾尔族组患者135例.NO、TNOS的表达水平汉族组患者分别为(134.24±27.60)μmol/L、(33.01 ±4.23) U/mL,维吾尔族组患者分别为(97.10±17.41)μmol/L、(27.41 ±3.95)U/mL,汉族组患者血清 NO、TNOS、iN-OS、eNOS含量均高于维吾尔族组患者,差异有统计学意义(P<0.01).ATDLI 汉族组患者血清NO、TNOS含量男性高于女性(P<0.05);维吾尔族组患者血清 NO、TNOS、iNOS 含量男性高于女性(P<0.01).血清 NO、TNOS、iNOS表达量同性别相比汉族组均高于维吾尔族组患者(P<0.001).汉族组、维吾尔族组不同BMI组间患者NO、TNOS含量比较,差异均有统计学意义(P<0.01);两民族中偏胖组患者NO、TNOS含量均高于偏瘦组和正常组(P<0.05).相关性分析表明,汉族组、维吾尔族组 NO 含量均与 BMI 值呈正相关(r值分别为0.444、0.677,P<0.01).汉族组、维吾尔族组不同肝损伤程度患者血清 NO、NOS 含量比较,差异均有统计学意义(P<0.05);两民族中轻度肝损伤组患者均低于中度与重度肝损伤组(P<0.001).结论 ATDLI汉族组与维吾尔族组患者血清NO及NOS含量在两民族之间存在差异,血清NO含量与BMI相关,且可随着肝损伤严重程度的增加而升高.%Objective To explore the expression levels of serum nitric oxide(NO)and nitric oxide synthase(NOS) between Han and Uyghur nationality patients with anti-tuberculosis drug-induced liver injury(ATDLI). Methods Patients with confirmed ATDLI in Chest Hospital of Xinjiang Uyghur Autonomous Region and First Affiliated Hos-pital of the Medical College of Shihezi University between January 2015 and May 2016 were chosen and divided into Han group and Uyghur group.By detecting the expression levels of NO and NOS in serum of ATDLI patients,ex-pression levels of serum NO and NOS in ATDLI patients of different gender,body mass index(BMI),and liver function injury were compared.Results 100 ATDLI patients in Han group and 135 in Uyghur group were recruited in study. Expression levels of NO and TNOS in Han group were(134.24±27.60)μmol/L and(33.01 ±4.23)U/mL respectively,in Uyghur groups were(97.10±17.41)μmol/L and(27.41 ±3.95)U/mL respectively,serum levels of NO,TNOS,iNOS,and eNOS in Han patients were all higher than Uyghur patients,difference was statistically significant(P<0.01). In Han ATDLI group,serum levels of NO and TNOS in male patients were both higher than female patients(P<0.05);in Uyghur ATDLI group,serum levels of NO,TNOS,and iNOS in male patients were all higher than female patients(P<0.01). The expression levels of serum NO,TNOS,and iNOS of Han group were all higher than the same gender in Uyghur group(P<0.001),difference in levels of NO and TNOS among different body mass index(BMI)groups in Han and Uyghur patients were both statistically significant(P<0.01). In both group,levels of NO and TNOS in obese patients were both higher than lean patients and normal weight patients(P<0.05). The correlation analysis showed that NO levels of Han and Uyghur groups were both positively correlated with BMI(r=0.444,0.677,respectively,P<0.01). There were significant differences in serum NO and NOS levels between Han and Uyghur patients with different degrees of liver injury(P<0.05);NO and NOS levels in both groups with mild liver injury were both lower than those with moderate and severe injury (P<0.001).Conclusion Serum NO and NOS levels between ATDLI Han group and Uyghur group are different,serum NO level is related to BMI,and it can increase with the degree of liver injury.