孕期染毒壬基酚对子代雄性胎鼠睾丸发育和胰岛素样因子-3表达的影响

摘要

Objective To investigate the effect of nonylphenol (NP) exposure on the genital development of fetal male rats in pregnant rats,and to measure the mRNA and protein expression of insulin-like factor 3 (Insl-3) in the testicular tissue of fetal rats.Methods A total of 100 pregnant SD rats were equally assigned to blank control group and four NP treated groups.Each rat in the NP treated groups received intragastric administration of NP at doses of 5,40,100,or 200 mg/kg/d from day 14 to 19 of gestation,and the rats in the blank control group received intragastric administration of pure peanut oil.The pregnant rats were sacrificed on day 19 of gestation.The body weight and testicular weight of each fetal rat were measured,and the descent of testis was also observed.The mRNA and protein expression of Insl-3 in the testicular tissue of fetal rats was analyzed by reverse transcription-PCR and Western blot.Results Compared with the blank control group,the 40,100,and 200 mg/kg NP treated groups showed significantly decreased body weight and weight coefficient of testis (P＜0.05 or P＜0.01),significantly decreased testicular descent (P＜0.05),and significantly decreased mRNA and protein expression of Insl-3 (P＜0.05 or P＜0.01).Conclusion Exposure to nonylphenol can lead to testicular maldevelopment,incomplete testicular descent,and Insl-3 expression downregulation of fetal male rats in pregnant rats.%目的 探讨母体染毒壬基酚(NP)对雄性胎鼠生殖发育的影响,并检测胎鼠睾丸组织中胰岛素样因子-3(Insl-3)的mRNA以及蛋白的表达.方法 将已受孕的SD雌性大鼠随机分为对照组、5、40、100、200 mg/kg NP染毒组(每组20只),染毒组大鼠于孕14～19 d以NP灌胃染毒,对照组大鼠用纯花生油灌胃第19天处死孕鼠后检测孕鼠和雄性胎鼠生殖发育相关指标；反转录-聚合酶链式反应(RT-PCR)和蛋白免疫印迹技术(Western blot)检测其睾丸组织中Insl-3的mRNA及蛋白的表达.结果 与对照组比较,40、100、200 mg/kg组胎鼠体重和睾丸系数明显降低,差异有统计学意义(P＜0.05或P＜0.01).与对照组比较,40、100、200 mg/kg组的睾丸下降程度降低,差异有统计学意义(P＜0.05).与对照组比较,40、100、200 mg/kg组Insl-3mRNA及蛋白表达降低,差异有统计学意义(P＜0.05或P＜0.01).结论 孕期暴露NP可致其雄性胎鼠睾丸发育不良,下降不全,并且可下调Insl-3的表达.