CEA重组痘苗病毒免疫治疗CEA阳性肿瘤机制的研究

摘要

目的：探讨癌胚抗原重组痘苗病毒(rV -CEA)治疗癌胚抗原(CE A)阳性肿瘤的机制。方法：使用我室构建的rV-CEA腹腔接种供体C57/BL小鼠后取其脾细胞 过继转移给经60Coγ射线亚致死量照射的荷CEA+-HePa肝癌细胞的C57/BL小鼠 ，并同时检测该供体小鼠脾细胞体外杀瘤细胞效应。结果：接种了rV-CEA的供体小鼠的脾细胞过继免疫给受体，具有明显 抑制CEA阳性肿瘤细胞生长的作用。结论：本研究提示rV-CEA对CEA阳性肿瘤的抑制作用可能主要是通过CE A特异的免疫反应激活T细胞而实现的，其机制值得进一步研究。%Objective:To explore antitumor mechanism of a re combinant vaccinia virus expressing human carcinomembryonic antigen(rV-CEA).[ WT5”HZ Methods:C57bl/6 mice were immu nized three times with rV-CEA.Six weeks later,the splenocytes from rV-CEA-imm unized donors were adoptively tranfered in sublethally irradiated CEA+-HePa- bearing recipients,meanwhile the antiumor effect of the donor splencytes in virt ro was detected.Results:Splenocytes from rV-CEA-immunize d donors expressed strong antiumor activity in CEA positive tumorbearing recipie nts,whereas W-VV-immunized and nonimmune donor cell did not.The antiumor acti vity was abolished when immune T cells were depleted before splencytes transfer.Conclusion:It was demonstrated that antiumor acivity induc ed by rV-CEA was essentially mediated by immune T cells which were activated by CEA-specific immune response.Adoptive immunotherapy strategies may have implic a tions for both the study of rV-CEA and the development of potential clinical a pplications for tumor immunotherapy.