人胸腺基质淋巴细胞生成素(hTSLP)在早孕绒毛组织的表达及意义

摘要

目的:探讨人早孕母胎界面表达TSLP及其受体(TSLPR)的特征.方法:使用RT-PCR、Western blot、免疫组织化学法分析正常绒毛组织TSLP/TSLPR的表达;RT-PCR、免疫细胞化学法分析原代培养的滋养细胞TSLP/TSLPR的表达;ELISA检测原代培养的人滋养细胞上清TSLP分泌水平.结果:从转录水平至蛋白水平,绒毛细胞滋养细胞与合体滋养细胞均表达TSLP及TSLPR.结论:TSLP表达于正常早孕绒毛组织,可能与早孕期调节性T细胞的扩增及功能变化相关.%Objective:To investigate the expression of a new cytokine,thymic stromal lymphopoietin (TSLP) and its receptor TSLPR in the villi of human first trimester gestation.Methods:Villi were collected from women who had undergone an artificial abortion at 7-11 weeks of normal gestation.The trophoblast cells (Tros) were isolated and cultured.The total RNA was extracted using TRIzol reagent,from both villi and the Percoll-gradient-isolated Tros,then the DNA fragments of hTSLP and hTSLPR were amplificated by RT-PCR.Villous tissues were detected for TSLP by immunohistochemistry (IHC) and Western blot.Immunocytochemistry (ICC) was carried out on cultured trophoblast cells for TSLP/TSLPR expression.Levels of TSLP in the supernatants were detected by ELISA.Results:Normal villi and the cultured Tros transcript were found to express TSLP/TSLPR mRNA and secreted TSLP protein.In addition,TSLP receptor was also expressed on trophoblasts.Conclusion:Both TSLP and its receptor are expressed on villi and trophoblast cells,which suggests that TSLP plays an important role in maternal-fetal immuno-tolerance in human early pregnancy.