中国HIV感染长期不进展者T细胞表面IL-7受体表达研究

摘要

Objective: To investigate the expression of Interleukin-7 (IL-7) receptor on T lymphocytes of Long-Term Non-Progressors (LTNP)and its association with disease progression. Methods: LTNP, HIV, AIDS patients and healthy controls (20 patients for each group)were enrolled and flow cytometry was used to detect the IL-7 receptor expression on T lymphocytes of whole blood samples taken from the patients and the controls. The association between IL-7 receptor expression on T lymphocytes and IL-7 level in plasma was analyzed. Results: The expression of IL-7 receptor on CD4 and CD8 + T lymphocytes and central memory T cells (Tcm), effector memory T cells (Tem) of LTNP group was significantly higher than that of AIDS and HIV groups ( P ＜ 0.05) ;The expression of IL-7 receptor on Na(i)ve and Terminally differentiated effector memory T cells(Tem/td) of LTNP group was not statistically different from that of AIDS, HIV groups and healthy controls. The percentage of IL-7 receptor expression on CD4, CD8+ T cells of H1V/AIDS patients negatively correlated with IL-7 level in plasma and HIV virus load and positively correlated with CD4 absolute value ( P ＜ 0.05) .Conclusion:The expression of the IL-7 receptor on T lymphocytes from peripheral blood is significantly correlated with HIV disease progression and higher level of IL-7 receptor expression on T cells of LTNPs may play a role in slowing down the disease progression.%目的:了解中国HIV感染长期不进展者(Long-Term Non-Progressor,LTNP)T细胞表面IL-7(Interleukin-7,IL-7)受体的表达,分析其与疾病进展的关系.方法:收集LTNP组、HIV组、AIDS组及健康对照组(每组各20例)的抗凝全血,用流式细胞仪检测IL-7受体的表达,并分析IL-7受体表达与血浆中IL-7水平的相关性.结果:LTNP组CD4+T、CD8+T、中枢记忆T细胞 (Central memory T cells,Tcm)、效应记忆性T细胞 (Effector memory T cells,Tem)表面IL-7受体表达水平明显高于无症状HIV感染组(HIV组)、AIDS病人组(AIDS组)(P＜0.05),LTNP组初始T细胞(Nave T cells,Nave)、终末分化效应记忆型T细胞(Terminally differentiated effector memory T cells,Tem/td)表面IL-7受体表达水平与HIV组、AIDS组间差异无统计学意义.HIV/AIDS患者IL-7受体在CD4+T和CD8+T细胞上表达的百分率与血浆IL-7水平呈明显负相关(P＜0.05),与CD4+T细胞数量呈明显的正相关(P＜0.05),与病毒载量呈明显负相关(P＜0.05).结论:外周血T细胞表面IL-7受体表达与HIV疾病进展密切相关,LTNP组T细胞保持较高水平的IL-7受体表达,可能是保护因素之一.