Objective: To investigate the positively relations between the efficacy of Chinese Medicine in the prevention influenrnza and mucosal immune. Methods: (1) Effect of several different preventive herbs on mucosal immunity in normal mice : Normal BALB/c mice were divided into different groups randomly , Blank control group , Ribavirin group ( Oral administration, 100 mg/kg) , Yinqiao Powder Preparation , Jade Screen Powder Preparation , Bing-xiang Powder Preparation ( Nebulised inhalation and Nasal drops ). All trial drug groups were administrated to mice 12 d. On the 12th day, mice were measured to collect the sera , rinse solution of intes— timal, nasopharynx, lung and the IgA antibodies in them were detected by ELISA , and Blank control group weighted the body and lung to measure the pulmonary index. (2) Effect of several different preventive herbs on mucosal immunity in mice infected with influenza virus: BALB/c mice infected with influenza were divided into six groups randomly , Virus control group , Ribavirin group, Yinqiao Powder Preparation, Jade Screen Powder Preparation , Bing-xiang Powder Preparation ( Nebulised inhalation and Nasal drops ). All trial drug groups were administrated to mice 7 d before infection. On the 5th day post-infection , mice were measured to collect the sera, rinse solution of intestimal, nasopharynx, lung and the IgA antibodies in them were detected by ELISA , and weighted the body and lung to measure the pulmonary index. Results: (1) Effect of several different preventive herbs on mucosal immunity in normal mice :rnPulmonary-index and virus titer decreased significantly ( P < 0. 01 ) in these groups of Ribavirin , Yinqiao Powder Preparation , Jade Screen Powder Preparation and Bing -xiang Powder Preparation by administration 7 d before infection, inhibition ratios of pulmonary -index were 25.46% up to 41. 61%. Compared with the Blank control group , the level of sera IgA in the Yinqiao Powder Preparation group and Bing-xiang Powder Preparation by nebulised inhalation were significantly increased after the treatment . The level of nasopharynx slgA in the Bing-xiang Powder Preparation by nasal drops was markedly increased . While the level of lung slgA in the Ribavirin was obviously decreased. The level of secretory IgA ( sIgA) in the rinse solution of intestimal descents after the treatment of Yinqiao Powder Preparation, Jade Screen Powder Preparation and Bing -xiang Powder Preparation. ( 2 ) Effect of several different preventive herbs on mucosal immunity in mice infected with influenza virus : Compared with the Virus control group , the level of Sera IgA in the Ribavirin group was obviously decreased , while markedly increased in the Bing-xiang Powder Preparation by nebulised inhalation group. The level of lung IgA was obviously decreased , but markedly increased in Jade Screen Powder Preparation group and Bing -xiang Powder Preparation by nebulised inhalation group including the level of slgA in the rinse solution of intestimal . Conclusion: Ribavirin has a inhibitory effect on mucosal immune in long -term treatment on normal and infected with mice at dosage of 100 mg/kg, so it should be considered that it is not recommended as preventive drugs of influenza . Three kind of medication type of Chinese Medicine in pre -vent influenza include oral administration, smoke inhalation and wearing aromatic drug. They could act on nasal mucosa, respiratory tract and digestive mucosa to stimulate local mucosal immune and produce secretory type IgA for the prevention and treatment of influ — enza. The effect of Chinese Medicine in the prevention influenza is related with mucosal immune .%目的:研究中药预防流感的作用与黏膜免疫相关性.方法:(1)不同预防方药预防给药对正常小鼠黏膜免疫的影响:将BALB/c小鼠随机分6组.设空白对照组 (生理盐水)、利巴韦林组、银翘散制剂、玉屏风散制剂、冰香散滴鼻组、冰香散雾化组.给药第12天,杀剖小鼠,空白对照组称体重和肺重测定肺指数.各组均采血分离血清,收集鼻咽冲洗液、支气管肺泡灌洗液、肠道冲洗液进行ELISA法检测IgA含量.(2)不同预防方药预防给药对流感病毒感染小鼠黏膜免疫的影响:将BALB/c小鼠随机分6组,设病毒对照组及药物试验组(同前).试验药物在滴鼻感染15 LD50流感病毒液感染攻击前提前给药一周,攻击后继续给药5天.共给药12天,每天一次给药.感染后5天,杀剖小鼠,称体重和肺重测定肺指数.采血分离血清,收集鼻咽冲洗液、支气管肺泡灌洗液、肠道冲洗液进行ELISA法检测IgA含量.结果:(1)不同预防方药预防给药对正常小鼠黏膜免疫的影响:连续给予正常小鼠12天试验药物,利巴韦林、银翘散制剂、玉屏风散制剂、冰香散对流感病毒鼠肺指数均有显著的改善作用,肺指数抑制率达到25.46%~41.61%.与空白对照组相比,银翘散制剂口服与冰香散雾化给药血清IgA水平显著提高;冰香散滴鼻给药鼻咽部sIgA水平显著提高;利巴韦林组给药支气管-肺泡sIgA水平下降;银翘散、玉屏风散、冰香散给药肠道sIgA水平有一定的提升作用,其中玉屏风散制剂作用明显.(2)不同预防方药预防给药对流感病毒感染小鼠黏膜免疫的影响:病毒感染小鼠攻击前预先连续给药7天,病毒感染第5天,与病毒对照组相比,利巴韦林给药血清IgA水平显著降低、冰香散雾化给药血清IgA水平显著提高;冰香散滴鼻给药能使鼻咽部sIgA水平显著提高;利巴韦林组给药支气管-肺泡sIgA水平下降,玉屏风散与冰香散雾化给药支气管-肺泡sIgA水平显著提高;玉屏风散制剂与冰香散雾化给药肠道sIgA水平显著提高.结论:利巴韦林长期预防给药(100 mg/kg)对黏膜免疫IgA分泌有一定抑制作用,不宜推荐作为流感病毒的预防性长期用药.中药预防流感的三种给药方式:口服、烟薰、芳香佩戴等均可作用于呼吸道或消化道黏膜,激发局部黏膜免疫产生分泌型IgA,从而起到预防和治疗的效果.中药预防流感的作用与黏膜免疫有一定的相关性.
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