OBctivte:To study the preventive and therapeutic effects of AromaticChinese herbs (ACH) on IFV-infected Madin-Darby canine kidney (MDCK) cellsand IFV-infected mice.rn Methods:The antiviral activity of nontoxic-level ACH against IFVA/FM/1/47 was examined by assaying (neutralization assay) its inhibitionof the IFV-induced cytopathic effects. The mode of ACH action was examinedwith a time-of-addition assay of synchronized infections,followed byviral attachment and penetration assays.MTT assay was used to evaluatemedian inhibitory concentration (ECM) and therapeutic index (TI).Inaddition,lung virus titers,lung index,lung histology,IFV load in lung,and the effect of ACH on pathogen recognition receptors of tracheal mucosawere detected.The inhibition of viral replication was demonstrated byquantitative real-time PCR,pathology technique and cell culturetechnique.rn Conclusions: Our results give scientific support to the use of preventivetreatment with ACH against influenza virus.ACH (intranasal and aerosolroute) has potential utility in preventing and controlling seasonalpandemics of influenza virus infections.
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