云芝糖肽对人PBMC中TLR5信号通路调控机制的研究

摘要

Objective: To detect the effects of polysaccharopeptide(PSP) on TLR5 signaling pathway in human PBMC,and explore the immunoregulatory mechanism of PSP. Methods: The human PBMC was isolated and cultured in vitro and then divided into control group and PSP group. Real time fluorescent quantitative PCR (Q-PCR)was used to quantitatively analyze the expression of the genes of TLR5 signaling pathway in different groups. Results: The expression levels of TLR5, IRAK4, TRAF6, NF-kB and IRF5 in the PSP group were significantly higher than these in the control group except the AP-1 (P <0. 05 ). Conclusion; PSP could play a role of regulation with the expressions of NF-kB and IRF5 mRNA in the TLR on the PBMC, and could supply evidences for further exploring the immunoregulatory mechanism of PSP.%目的:研究云芝糖肽(PSP)对人外周血单个核细胞(PBMC)中TLR5信号通路的调控作用,探索PSP对Toll样通路的免疫调节机制.方法:体外分离、培养人PBMC,分为对照组和PSP处理组,采用实时荧光定量PCR (Q-PCR)技术对PSP处理人PBMC前后,PBMC中的TLR5信号通路中相关基因的表达差异情况进行定量分析.结果:PSP处理的实验组与对照组相比较,TLR5信号通路中的TLR5、IRAK4、TRAF6、IRF5和NF-κB基因的表达水平均显著增高(P＜0.05),但AP-1基因的表达降低(P＜0.05).结论:PSP对PBMC中TLR通路的调控可能主要通过NF-κB和IRF5调控终端效应因子来发挥免疫调节作用,这些为进一步探讨PSP的免疫调节作用机理提供了实验依据.