Th17细胞在实验性自身免疫性脑脊髓炎大鼠发病中的表达及依达拉奉的保护作用研究

摘要

目的:探讨Th17细胞在实验性自身免疫性脑脊髓炎(EAE)大鼠发病中的动态变化及依达拉奉可能的保护作用机制.方法:对比EAE组和依达拉奉组大鼠发病前期、急性期和缓解期Th17相关细胞因子.评估大鼠临床发病情况；组化染色观察脊髓组织炎症细胞中RORγt阳性表达；ELISA法检测血清中IL-17、IL-6含量；实时定量PCR检测脊髓组织IL-17mRNA、HO-1mRNA含量.结果:依达拉奉组平均发病时间、发病率、急性期神经功能评分明显低于EAE组(P＜0.05)；免疫组化结果显示依达拉奉组各时期RORγt阳性细胞平均秩次均低于EAE组(P ＜0.05)；ELISA结果显示依达拉奉组发病前外周血中IL-17、IL-6的含量低于EAE组(P＜0.05),在急性期和缓解期依达拉奉组外周血中IL-17均含量低于EAE组(P＜0.05)；实时定量PCR发现依达拉奉组急性期IL-17mRNA表达低于EAE组(P＜0.05),依达拉奉组不同时期HO-1mRNA含量均高于EAE组(P＜0.05).结论:依达拉奉可延迟EAE发病时间、减轻神经功能损伤、降低发病率；可能通过作用于HO-1起到抗氧化应激和抗炎作用.%Objective: To study the expression of Thl7 in EAE and to explore the effect and the underlying mechanism of Edar-avone on EAE. Methods: EAE rats were established and some rats were interfered with Edaravone randomly. There were three disease phases (Pre-disease, Acute Phase, Remission Stage) were observed. The clinical syndrome were assessed first; then positive cells of RORγt in spinal cord were observed by immunohistochemistry; the content of IL-6 and IL-17 in peripheral blood were detected with ELISA; the expression of IL-17mRNA and HO-lmRNA were determined by RT-PCR. Results;The decrease of morbidity and clinical neurological score were more obvious in Edaravone group than in EAE group (P < 0. 05). Expression of RORγt and content of IL-6 and IL-17/IL-17mRNA were lower in Edaravone group than in EAE group(P <0.05). Content of HO-lmRNA in Edaravone group was higer than in EAE group. Conclusion;Edaravone could alleviate clinical severity of EAE rats; it may play its anti-oxidative and anti-inflammatory roles through acting on HO-1.