马齿苋提取物对急性湿疹大鼠皮肤TNF-α与IL-4表达的影响

摘要

目的：探讨马齿苋提取物对急性湿疹大鼠背部皮肤TNF-α与IL-4表达的影响及其机制。方法：SD大鼠40只，随机分为4组，每组10只，分别为正常组、模型组、复方黄柏组、马齿苋组。除正常组外其余各组用2，4-二硝基氯苯（ DNCB）于大鼠右背部皮肤造模，造模成功后，模型组、复方黄柏组、马齿苋组分别给予1 ml无菌蒸馏水、复方黄柏液和马齿苋提取液。实验第21天断颈处死动物，肉眼观察4组大鼠右背部皮肤病变，取大鼠左、右背部相同面积皮肤，称重法比较皮肤重量，免疫组化法（ SP法）染色4组大鼠右背部皮肤TNF-α、IL-4并采用病理图像分析系统半定量检测其含量。结果：正常组大鼠右背部皮肤光滑、未见病理性改变，模型组大鼠右背部皮肤出现明显肿胀、红斑、糜烂和渗出，复方黄柏组、马齿苋组上述病变均有不同程度减轻。与模型组比较，复方黄柏组和马齿苋组大鼠皮肤肿胀度明显减轻（ P＜0.01）；与复方黄柏组比较，马齿苋组大鼠皮肤肿胀度有所减轻（ P＜0.05）。空白组、模型组、复方黄柏组、马齿苋组的 TNF-α含量分别是（6652.66±1190.94）、（19927.10±5494.21）、（7515.13±877.66）、（6809.93±1385.54）；IL-4含量分别是（5378.44±1685.01）、（26334.89±3993.48）、（7814.84±1751.38）、（8246.57±975.08）。与模型组比较，复方黄柏组和马齿苋组大鼠皮肤组织TNF-α、IL-4含量显著降低（ P＜0.01）；与复方黄柏组比较，马齿苋组大鼠皮肤组织TNF-α、IL-4含量明显降低（ P＜0.05）。结论：马齿苋提取液对湿疹皮肤具有明显的疗效，其机制可能是通过下调促炎因子TNF-α，同时适度的调控抗炎因子IL-4发挥作用。%Objective:To investigate the influence and mechanism of Portulaca extracts on Tumor Necrosis Factor -α(TNF-α), Interleukin-4(IL-4) in skins of rats with atopic eczema (AE).Methods:40 SD rats were randomly divided into normal control group , model group,Cortex Phellodendri group and Porulaca group.2,4-dinitrochlorobenzene was used on the right back of rat for making atopic eczema.21 days later , all animals were sacrificed , compared the weight of the same size of the eczema and healthy skin.The levels of TNF-αand IL-4 in rats skins were detected by immunohistochemical method.Results:The right back of rats in model group appeared obvious erythema ,erosion and leakage ,which meant the modeling succeeded.Compared with model group ,the swelling degree of Cortex Phellodendri group and Purslane group were significantly decreased ( P<0.01 ) ,compared with Cortex Phellodendri group ,the swelling degree of Purslane group were less than them ( P<0.05 ).The levels of TNF-α, in rats skins of normal control group , model group,Cortex Phellodendri group and Portulaca group were (6 652.66±1 190.94 ),(19 927.10±5 494.21 ), (7 515.13±877.66 ), (6 809.93±1 385.54).The levels of IL-4 were (5 378.44±1 685.01),(26 334.89±3 993.48),(7 814.84±1 751.38),(8 246.57± 975.08 ).Compared with model group ,the levels of TNF-α,IL-4 in rats skins of Cortex Phellodendri group and Purslane group were sig-nificantly decreased(P<0.01).Compared with positive drug group,the levels of TNF-α,IL-4 in rats skins of Purslane group were a little decreased(P<0.05).Conclusion:Purslane extracts has effect to treat atopic eczema ,its possible mechanism is by erducing the proinflammatory factor ,and moderating the anti-inflammatory factor and the ratio between them.