IL-17在类风湿关节炎患者外周血表达的临床意义

摘要

Objective:To explore the role and significance of IL-17 on the pathogenesis of rheumatoid arthritis ( RA).Methods:The levels of IL-17 mRNA in peripheral blood mononuclear cell ( PBMC) of 66 RA patients (32 active,34 inactive) and 44 healthy controls were detected by reverse transcription-polymerase chain reaction ( RT-PCR ).The levels of serum IL-17 were detected by enzyme-linked immunosorbent assay ( ELISA).The levels of RF were detected by immunoturbidimetric assay.The correlations of serum IL-17 and RF were analyzed.Results: In RA patients the levels of IL-17 mRNA in PBMC were significantly higher than those in healthy controls ( P<0.05 ) , but there was no difference between the active and inactive RA ( P>0.05 ).The serum IL-17 levels in active RA patients were significantly higher than those in inactive RA patients and healthy controls ( P<0.05 ).The levels of serum IL-17 were positively correlated with RF in RA patients.Conclusion: IL-17 and RF play an important role in the pathogenesis and pathology process of RA ,and IL-17 may be associated with RA activity.%目的：探讨IL-17在类风湿关节炎（ RA）发病中的作用及意义。方法：采用逆转录-聚合酶链反应（ RT-PCR）法检测66例RA患者（活动期34例、缓解期32例）和44例健康对照组外周血单个核细胞（ PBMC）中IL-17 mRNA 的表达水平，采用酶联免疫吸附试验（ ELISA）检测各组血清中IL-17的浓度，用免疫比浊法检测各组血清RF的浓度，分析血清IL-17与RF的相关性。结果：RA患者PBMC中IL-17 mRNA的表达显著高于健康对照组（ P＜0.05），但活动期与缓解期无明显差异（ P＞0.05）；RA活动期患者血清IL-17水平显著高于RA缓解期患者和健康对照组（ P＜0.05）；且RA患者血清IL-17水平与RF呈正相关。结论：IL-17和RF在RA的发病和病理过程中起重要作用，IL-17可能与RA活动性有关。