MicroRNA-150通过降低CD122的表达调控NK及NKT细胞的分化

摘要

To explore the effects of microRNA-150 deletion on the development and homeostasis of regulatory T cells (Treg),γδT cells,NK and NKT cells.Methods:microRNA-150 knockout mice were used and microRNA-150 expression was detected by Real-time PCR.The numbers of Treg ,γδT,NK and NKT cells in the thymus and spleen of normal control and microRNA-150 knockout mice were detected by Flow cytometry.Cell apoptosis was detected by Annexin V staining , and cell proliferation was detected by 5-Bromo-2-deoxyUridine ( Brdu ) incorporation.Results: microRNA-150 deletion did not affect the development and homeostasis of regulatory T cells (Treg) andγδT cells.However,microRNA-150 deletion resulted in a significant reduction of the NK and thymic NKT cell number.In addition, microRNA-150 deleted NK and NKT cells showed an arrested developmental and maturational phenotype with a reduced expression of NK 1.1 and CD122.Moreover , cell apoptosis was significantly increased in microRNA-150 deleted NK and thymic NKT cells ,while a lower cell proliferation rate was shown in the microRNA-150 deleted NK but not NKT cells.Conclusion: CD122 may play an important role in the development and homeostasis of mouse NK and NKT cells regulated by microRNA-150.%目的：探讨microRNA-150缺失对调节性T细胞（ Treg）、γδT细胞、NK及NKT细胞产生、发育和自稳的影响。方法：采用microRNA-150基因敲除小鼠；Real-time PCR检测microRNA-150的表达；流式细胞术检测小鼠胸腺和脾脏Treg、γδT细胞、NK及NKT细胞数量变化；采用Annexin V染色法检测细胞凋亡；5-溴脱氧尿嘧啶核苷（Brdu）掺入法检测细胞增殖。结果：microRNA-150缺失对小鼠Treg 和γδT细胞的产生和发育不发挥明显作用，但导致NK和胸腺NKT细胞数量显著降低，而且，microRNA-150缺失导致小鼠NK及NKT细胞中CD122的表达显著降低，细胞发育受阻，凋亡增加，同时NK细胞的增殖能力也显著降低。结论：microRNA-150调控小鼠NK和NKT细胞的发育和自稳，CD122可能在以上调控过程中发挥重要作用。