水红花子对 LPS 诱导流产小鼠子宫巨噬细胞的抑制效应及其保胎作用

摘要

目的：探讨水红花子对细菌脂多糖（ LPS）诱导流产小鼠的免疫抑制及其保胎作用。方法：昆明种小鼠55只，随机分为对照组（A组，10只）和实验组。实验组又分为细菌脂多糖（LPS）处理组（B组），水红花子处理组（C组），水红花子及LPS双处理组（ D组），每组各15只。观察妊娠结果，用酶组织化学、免疫组化和ELISA方法分别检测非特异性酯酶阳性（α-NAE＋）、CD14＋和CD204＋巨噬细胞、TNF-α的变化。结果：B组小鼠流产率和胚胎吸收率均为100％（ P＜0.01）；而D组流产率降低为13.33％，胚胎吸收率降至10.39％。与A组相比，在B组，α-NAE＋、CD14＋巨噬细胞数量和阳性面积，以及TNF-α含量均显著增加；在D组，环肌外侧极显著增多（P＜0.01），但环肌内侧、功能层均接近正常水平。与A、B组相比，C、D组CD204＋巨噬细胞数量和阳性面积均显著增多。结论：通过调节子宫巨噬细胞的表型、活性和TNF-α的分泌，可能是水红花子对抗LPS所致流产效应的机制之一。%Objective:To explore the immunosuppression effects and tocolysis of fructus polygoni orientalis on abortion mice induced by LPS.Methods:Mice of Kunming (55 mice) were divided into control group (group A ,10 mice) and experimental group. Experimental group were divided into group B ( intraperitoneal injection LPS ) , group C ( ingestion fructus polygoni orientalis ) , and group D (ingestion fructus polygoni orientalis and intraperitoneal injection LPS ),each group of 15 mice.Then the pregnancy results were observed ,the positive of α-NAE+and the varity of CD 14+and CD204+macrophages ,TNF-αin the uteri were identified by enzyme-histochemistry ,immunohistochemistry and ELISA.Results:The abortion rate and the embryo resorbing rate were all 100%( P<0.01 ) in group B.But there was the decreased abortion rate of 13.33% in group D .The embryo resorbing rate decreased to 10.39%.The number and positive cell area of α-NAE+and CD14+macrophages in the uteri of gestation mice of group B was greatly increased comparing with group A ( P<0.01 ) .These effects outside of myometrium of group D were remarkably increased comparing with group A (P<0.01),but there was no distinct difference in the inside of myometrium and function layer.The number and positive cell area of CD204+macrophages in group C and D was greatly increased comparing with group A and B ( P<0.01 ) .The TNF-αcontents in the uteri of mice in group B were greatly increased comparing with group A (P<0.01),but the positive cell area of CD14,CD204 were close to normal levels in group D.Conclusion:The effect of miscarriage induced by LPS is antagonized by fructus polygoni orientalis through inhibiting the phenotype ,activity and function characteristics of macrophages in the uteri of gravidity mice.