Musashi 1在结肠癌中的表达及其与微血管密度及预后的关系

摘要

目的：探讨Musashi 1在结肠癌中的表达与肿瘤微血管密度（ MVD）及预后的关系。方法：应用实时荧光定量PCR的方法检测36对结肠癌组织及其配对的正常结肠组织中Musashi 1 mRNA的表达情况。通过免疫组化法对96例结肠癌组织中Musashi 1表达情况和微血管密度（ MVD）进行检测。结合随访术后生存情况，统计分析Musashi 1表达与结肠癌病理参数、预后及MVD之间的相关性。结果：实时荧光定量PCR法结果显示，在结肠癌组织中，Musashi 1 mRNA的相对表达水平显著高于正常结肠组织（P＜0．05）。免疫组化结果显示，96例结肠癌组织中，有61例（63．5％）高表达Musashi 1。 Musashi 1的表达与患者性别、肿瘤大小、分化程度无相关性（P＞0．05），而与肿瘤的浸润深度、Dukes分期、淋巴转移、TNM分期密切相关（ P＜0．05）。 Kaplan－Meier生存分析显示，Musashi 1高表达患者的5年生存率显著低于Musashi 1低表达者（23．0％vs 60．0％；P＜0．01），且Musashi 1的表达与结肠癌血管密度相关（P＜0．01）。结论：结肠癌中Musashi 1的表达较正常癌旁组织高，具有统计学意义。结肠癌中Musashi 1的表达与肿瘤浸润深度、Dukes分期、淋巴转移、TNM分期和血管密度密切相关。 Musashi 1蛋白可通过促进瘤内血管增生，从而促进结肠癌的生长和侵袭转移，进一步影响结肠癌患者的预后。%Objective:To investigate the Musashi 1 expression, and its correlation with clinical variables, such as clinical pathologic factors,angiogenesis and prognosis in colon cancer.Methods: Musashi 1 mRNA level was determined by quantitative real-time PCR( qRT-PCR) in 36 pairs of matched colon cancer tissues and adjacent non-tumorous tissues.The expression of Musashi 1 and mi-crovascular density ( MVD) were tested by immunohistochemical in 96 cases of colon cancer.Combined with postoperative follow-up survival situation, the correlation between the expression of Musashi 1, pathological parameters, prognosis and MVD were statistical analylised.Results:Musashi 1 mRNA level of colon cancer tissues determined by PCR was significantly higher than adjacent non-tumorous tissues( P<0.05).Positive Musashi 1 immunoreactivity was seen in 63.5%of colon cancer specimens,which was correlated with the depth of invasion,Duckes stage,lymph node metastasis,and TNM stage (P<0.05).Compared with Musashi 1 negative patients, Musashi 1 positive patients had significantly shorter survival time ( 23.0% vs 60.0%, P<0.01 ) .High intratumor blood microvessel density patients had significantly shorter survival times than low intratumor blood microvessel density patients(P<0.05).Intratumor blood microvessel density correlated with Musashi 1 expression (P<0.01).Conclusion:Musashi 1 mRNA level of colon cancer tissues was higher than adjacent non-tumorous tissues,the difference has statistically significant.We conclude that Musashi 1 expression correlates with the depth of invasion,Dukes stage,lymph node metastasis,and TNM stage and microvascular density ( MVD) in colon cancer.We propose that synthesized Musashi 1 increases intratumor angiogenesis,thus promotes tumor progression.Musashi 1 expression may be a good biomarker for poor prognosis in colon cancer.