当归对阴虚哮喘小鼠气道MUC5 AC及相关炎症因子表达的影响

摘要

目的：探讨当归对阴虚哮喘BALB／c小鼠气道MUC5AC及相关炎症因子表达的影响。方法：取雌性小鼠随机分7组（n＝12），通过注射卵清白蛋白（OVA）致敏、吸入OVA激发复制哮喘模型，实验后期给予甲状腺素以复制阴虚哮喘BALB／c小鼠模型；在受试药的干预下，观测当归对哮喘行为学、肺组织病理学、BALF 中活性因子（包括 IL－13、TNF－α、MUC5AC）及肺组织中MUC5AC表达的影响。结果：2、4、8 g／kg当归可明显降低哮喘行为学综合评分，缓解肺组织病理学变化，降低BALF中IL－13、TNF－α及MUC5AC的水平，抑制肺组织中MUC5AC的表达（P＜0．05，0．01）。同时，当归与地塞米松配伍后抑制IL－13与MUC5AC表达有一定的协同作用（P＜0．05）。结论：当归对哮喘具有一定的治疗作用，通过抑制IL－13、TNF－α而调节MUC5AC的表达可能是其作用机制之一，而在调节IL－13与MUC5AC的表达方面当归与糖皮质激素有一定的协同作用。%Objective:To study the effects of Radix Angelicae Sinensis ( RASI) on expression of airway MUC5AC and related inflammatory factors in asthmatic mice with Yin deficiency syndrome.Methods:Injecting ovalbumin ( OVA) to sensitize,inhaling OVA to stimulate,using Thyroxin during late stimulation,the asthmatic mouse with Yin deficiency syndrome was established and evaluated through asthmatic behaviors, lung histopathology, active factors ( IL-13, TNF-αand MUC5AC ) in broncho-alveolar lavage fluid (BALF), and MUC5AC expression in lung tissue.Results: 2,4,8 g/kg RASI could reduce asthmatic behaviors score, relieve pathological changes of lung tissue,reduce the contents of IL-13,TNF-αand MUC5AC in BALF,and depress MUC5AC expression in lung tissue ( P<0.05,0.01).In addition,there was a certain synergy between RASI and dexamethasone ( DXM) on depressing the ex-pression of IL-13 and MUC5AC (P<0.05).Conclusion:RASI has certain anti-asthma effect and one of mechanisms is to regulate the MUC5AC expression through inhibit IL-13 and TNF-α.On the expression of IL-13 and MUC5AC,the compatibility of RASI with glu-cocorticoid has some synergy effect.