不育夫妇血清抗精子抗体和抗心磷脂抗体检验结果分析

摘要

Objectives:To explore the expression and significance of anti -sperm antibodies and anti -car-diolipin antibodies in serum of infertile couples.Methods:60 infertile couples and 60 healthy couples admitted in our hospital were chosen as observation group and control group respectively.Enzyme -linked immunosorbent assay was used to detect the level of anti -sperm antibodies and anti -cardiolipin antibodies.Semen for both groups were analyzed and compared.Results:The serum anti -sperm antibodies of male patients were significantly higher than control group (P <0.05),while difference in anti -cardiolipin antibodies was significantly different (P >0.05). The two antibodies in female patients of observation group were stronger than control group (P <0.05).The positive anti -sperm antibody had no effect on pH value,semen volume,sperm density and b grade sperm in both two groups,however,a grade sperm,sperm viability and curvilinear velocity were lower than control group (P <0.05). Conclusions:Patients with anti -sperm antibodies and anti -cardiolipin antibodies may affect the sperm viability and sperm motility.%目的：对不育夫妇血清抗精子抗体及抗心磷脂抗体进行检查，并分析其检测结果，以探讨两者在患者血清中的表达及意义。方法：选取本院所收治的60对不育夫妇作为观察组，同时选取同期来院进行健康体检的60对健康夫妇作为对照组，采用酶联免疫吸附试验法检测两组被检人员的抗精子抗体及抗心磷脂抗体情况。对两组人员精液情况进行分析和比较。结果：经检测研究发现，观察组男性患者血清抗精子抗体表达明显高于对照组（P ＜0.05）；而两组抗心磷脂抗体相比无明显差异（P ＞0.05）。观察组女性患者抗精子抗体及抗心磷脂抗体较对照组强（P ＜0.05）；男性抗精子抗体阳性对 pH 值和精液量及精子密度、b级精子均无影响。观察组患者 a 级精子和精子活率及曲线运动速度、直线运动速度较对照组低（P ＜0.05）。结论：患者抗精子抗体及抗心磷脂抗体可能影响其精子活率和精子运动。