Objective To study the expression of vascular endothelial growth factor (VEGF) and its receptor, Cyclin E and CDK2 in the liver of the rat liver cancer model, and to explore the function and significance of VEGF, VEGFR1, Cyclin E and CDK2 in the liver cancer progression. Methods Based on the DEN-induced hepatocarcinogenesis model, the expressions of VEGFR1, Cyclin E and CDK2 were detected with immunohistochemical staining, and the quantity of VEGF in the serum was detected with enzyme linked immunosorbent assay. Results The quantity of VEGF in the serum of rats is lowest in the control group, and increased gradually in the experimental groups. The average optic density values of VEGFR1,Cyclin E and CDK2 protein expression had increasing trends during the development of liver cancer. The quantity of VEGF in the rat serum and the average optic density values of VEGFR1, Cyclin E and CDK2 protein were positively correlated (r = 0. 834, F = 42. 1274 , P＜0. 05). Conclusion Abnormal expression of VEGF and its receptor, promotes hepatocarcinogenesis and tumor development, which might be related with the abnormal expression of Cyclin E and CDK2 protein.%目的 通过观察血管内皮生长因子(VEGF)及其受体和细胞周期蛋白E (Cyclin E)在肝癌模型大鼠肝脏中表达情况,探讨VEGF与细胞周期相关蛋白在肝癌发生发展过程中的作用.方法 建立诱发性肝癌模型,采用酶联免疫吸附试验检测血清中VEGF量的变化,免疫组化技术检测VEGFR1、Cyclin E和细胞周期蛋白依赖性激酶(CDK2)的表达情况.结果 血清中的VEGF含量在对照组中最低,在实验组中逐渐增多,以癌变期含量最高.VEGFR1、Cyclin E和CDK2蛋白表达的平均光密度值均随着肝癌的发生发展有增高的趋势,大鼠血清中的VEGF量与肝脏组织中VEGFR1、Cyclin E和CDK2蛋白表达的平均光密度值随着肝癌的发生发展呈正相关(r=0.834,F=42.1274,P<0.05).结论 VEGF及其受体VEGFR1在肝癌发生发展中异常表达,促进肝癌的发生发展,可能与Cyclin E、CDK2细胞周期蛋白异常表达有关.
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