大鼠PTSD后蓝斑核 β-catenin的表达下降伴随细胞凋亡

摘要

目的 探讨创伤后应激障碍( PTSD) 大鼠蓝斑神经元β-catenin(β-连环蛋白)的表达变化.方法 采用国际认定的SPS方法刺激建立大鼠PTSD模型,取成年健康雄性Wistar 大鼠100 只,随机分为连续单一刺激( single prolonged stress,SPS) 模型1 d、4 d、7 d、14 d 组和对照组,应用免疫组化、免疫印迹方法检测PTSD 大鼠蓝斑神经元β-catenin的表达变化;透射电镜观察PTSD大鼠蓝斑神经元的超微结构变化.结果 经SPS 刺激后大鼠蓝斑神经元细胞内β-catenin于1d开始逐渐减少,14d表达最少;蓝斑神经元出现细胞凋亡改变.结论 蓝斑神经元细胞凋亡可能是导致PTSD 患者蓝斑功能失调的重要原因之一.%Objective To investigate the expression of β-catenin in the locus coeruleus of rats with post-traumatic stress disorder ( PTSD) . Methods The SPS-method was used to set up the rat PTSD models. A total of 100 health , male Wistar rats were randomly divided into 1, 4, 7 and 14d groups of single prolonged stress ( SPS ) and a normal control group. Immunohistochemistry, immunofluorescence,and western blot were used to observe and detect the changes in the expression of β-catenin in the locus coeruleus neurons, while ultrastructure was detected by electron microscopy. Results Apoptotic cells were present in the locus coeruleus of PTSD rats. The expression of β-catenin in the locus coeruleus gradually decreased from 1d after SPS, and reached the minimum at SPS 14d. Conclusion The changes of β-catenin expression in the locus coeruleus may play important roles in the dysfunction of the locus coeruleus in PTSD patients