Objective To investigate the difference of hepatic microvessel density, neovasculariza-tion of regenerating liver tissue after ablation of two ways of irreversible electroporation and radiofrequency ablation in rats. Methods 90 male Sprague-Dawley rats were randomly divided into 3 groups, including the control group ( n =30), the irreversible electroporation group ( n =30 ) and the radiofrequency ablation group (n=30). 3,7 and 10 days were executed after the operation and draw material, expression of vascu-lar endothelial growth factor(VEGF) and CD34 in tissue was studied by immunohistochemistry, and the mi-crovascular density of tissue and VEGF positive cells were measured. Results The microvascular density of 3, 7 and 10 days in the control group was 50. 3 ± 12. 5, 54. 6 ± 11. 9 and 58. 2 ± 14. 7, the microvascular density of the radiofrequency ablation group was 18. 4 ± 4. 7, 17. 3 ± 5. 1 and 18. 1 ± 5. 9, respectively. The microvascular density of the irreversible electroporation group was 42. 8 ± 10. 4, 45. 6 ± 10. 2 and 49. 2 ± 13. 8, respectively. The positive cells of VEGF in control group was 50, 56 and 57 at 3, 7 and 10 days, and 32, 30 and 33 at 3, 7 and 10 days in radiofrequency ablation group, 44, 43 and 45 at 3, 7 and 10 days in irreversible electroporation group; expression of VEGF and CD34 in 3, 7, 10 d and the microvascular density of ablation area in radiofrequency ablation group was significantly lower than those in control group after irreversible electroporation and radiofrequency ablation. No significant differences were found between irreversible electroporation group and control group. Conclusion The irreversible electroporation can effectively protect the microvessels in the ablation area, ensure the tissue’s blood supply after the ablation, and provide a guarantee for the repair and regeneration of the tissue.%目的 比较不可逆电穿孔(纳米刀)技术与射频消融对大鼠肝脏新生血管的影响.方法 雄性Sprague-Dawley大鼠90只,采用随机数字表法分为对照组、纳米刀组和射频消融组,每组30只.纳米刀组电极消融肝脏左侧外叶,射频消融组消融相同部位,对照组仅开腹和关腹.术后3、7和10 d取消融区域标本,行免疫组化染色.检测血管内皮生长因子( VEGF)和CD34阳性表达,并计算微血管密度和VEGF阳性细胞比例.结果 术后3、7和10 d,对照组微血管密度计数分别为50. 3 ± 12. 5、54. 6 ± 11. 9 和58. 2 ± 14. 7,射频消融组为18. 4 ± 4. 7、17. 3 ± 5. 1 和18. 1 ± 5. 9,纳米刀组为42. 8 ± 10. 4、45. 6 ± 10. 2和49. 2 ± 13. 8.射频消融组微血管密度低于对照组及纳米刀组,差异有统计学意义(P＜0. 05).纳米刀组微血管密度与对照组比较,差异无统计学意义( P＞0. 05).术后3、7和10 d,对照组VEGF阳性细胞数分别为50、56和57个,射频消融组为32、30和33个,纳米刀组为44、43和45个.射频消融组VEGF阳性细胞比例低于对照组和纳米刀组,差异有统计学意义( P＜0. 05).纳米刀组与对照组VEGF阳性细胞比例比较,差异无统计学意义(P＞0. 05).结论 纳米刀可以有效保护大鼠肝脏消融区的微血管,消融区血管再生能力更强,优于射频消融,为组织修复及再生提供保障.
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