CD148在健康志愿者血小板表面表达的初步探讨

摘要

目的 测定CD148在健康志愿者血小板表面的表达情况,为以后研究CD148在血小板中的作用奠定基础.方法 选取31例健康志愿者静脉血,滴定CD148抗体获得饱和浓度,应用流式细胞仪分别测定31例志愿者血小板表面CD148的表达,4℃保存24h后7例志愿者血小板表面CD148的表达,以及血小板膜被穿孔后CD148的表达.结果 CD148的平均荧光强度明显高于阴性对照(8.32±3.37 vs 0.97±0.20),差异有统计学意义(P＜0.01),4℃保存24 h后较当天分析的血小板表面CD148表达的平均荧光强度有所下降(7.89±1.26 vs 9.17±2.35),但差异无统计学意义.CD148在被穿孔后血小板上的表达明显高于未被穿孔的血小板(14.95±0.72 vs 9.62±0.96),差异有统计学意义(P＜0.01).结论 人类血小板表面有CD148表达,在4℃保存24 h后CD148的表达有所下降,但影响不大,CD148在被穿孔后血小板上的表达明显高于未被穿孔的血小板.本方法简单易行,为进一步研究CD148在血小板中的作用奠定了基础.%Objective To determine the expression of CD148 on the platelet surface in healthy volunteers.Methods The blood sample of 31 healthy volunteers were collected.We titrated CD148 antibody to attain saturated concentration.CD148 expression on the surface of platelets in 31 volunteer were measured by flow cytometry.We tested the seven volunteers after 24 hours and looked at total CD148 by using permeabilized platelets.Results Median fluorescence intensity of negative control group and CD148 group are (8.32±3.37 vs 0.97±0.20)respectively.The difference was statistically significant between the two groups (P＜0.01).Median fluorescence intensity of day 0 positive group and day 1 positive group are(9.17±2.35 and 7.89±l.26)respeetively.The difference was not statistically significant between the two groups(P＞0.05).CD148 expressions on permeabilized platelets were increased compared with those on native platelets(9.62±0.96 vs 14.95±0.72,P＜0.01).Conclusions CD148 expresses significantly on the human platelet surface.There is no difference between CD148 expressions on platelets in day 0 and day 1.Day 1 CD148 expressions on platelets in some donors have decreased and some have increased, so we need further investigation.After being perforated, the expression of CD148 was significantly higher than that of not being perforated.This method provides a simple way to study the role of CD148 in platelet.