细脚拟青霉提取物对D-半乳糖致衰小鼠免疫功能的影响

摘要

Objective To study the effects of Paecilomyces tenuiqes extracts on immunoloregulation of D-galactose aging mice.Methods The aging model mice induced by D-galactose were given Paecilomyces tenuipes extracts with three different doses. 30 days later,the thymus index and spleen index were calculated, the spleen lymphocyte transforming stimulation index (SI) was measured with MTT. The phagocytosis of peritoneal macrophage was analyzed by neutral red test. The level of IL-2 was assayed by ELISA. Results The immunological function of D-galactose aging mice was decreased obviously. The phagocytosis of peritoneal macrophage was improved significantly by each dose (P ＜ 0. 01 ). The middle and high dose of Paecilomyces tenuipes extracts could increase significantly the thymus index, spleen index and IL-2 level (P＜0.01). SI of T and B lymphocyte were raised significantly by the high dose (P＜0.01). Conclusions Paccilomyces tenuipes extracts could regulate the immunological function of D-galactose aging mice.%目的 研究细脚拟青霉提取物对D-半乳糖致衰小鼠免疫功能的调节作用.方法 以D-半乳糖制备衰老小鼠模型,同时用低、中、高三种不同剂量的细脚拟青霉提取物进行灌胃30 d后,计算胸腺指数和脾脏指数,用MTT法检测小鼠脾T、B淋巴细胞转化指数SI,用中性红试验检测小鼠腹腔巨噬细胞吞噬功能,用ELISA法检测IL-2的水平.结果 D-半乳糖致衰小鼠免疫功能明显下降,各剂量组均能显著提高小鼠腹腔巨噬细胞吞噬功能(P<0.01);中、高剂量细脚拟青霉提取物能够显著提高胸腺指数、脾脏指数、IL-2水平(P<0.01);高剂量细脚拟青霉提取物能够显著提高脾T、B淋巴细胞转化指数SI(P<0.01).结论 细脚拟青霉提取物对D-半乳糖致衰小鼠免疫功能具有一定的调节作用.