目的 制备控释双生长因子海藻酸钠水凝胶载体,并检测其理化性能.方法 采用氧化及钴60辐照对海藻酸钠进行修饰,制备控释双生长因子海藻酸钠水凝胶载体.将无针注射器中海藻酸钠水凝胶打入24孔培养板中,通过测定水凝胶的干重损失检测控释双生长因子海藻酸钠水凝胶的体外降解.用无针注射器于18只兔心肌组织喷射100 μl双生长因子海藻酸钠水凝胶,分别于各时间点取心肌组织,进行HE染色,观察海藻酸钠水凝胶的体内降解情况.采用MTT法进行控释双生长因子海藻酸钠水凝胶的细胞毒性分析.结果 体外降解实验中,控释双生长因子海藻酸钠水凝胶显示了相对较快地降解,第1周大约降解50%,3 w后几乎降解完全.体内降解实验显示,心肌组织中控释双生长因子海藻酸钠水凝胶逐渐减少,5 w后,在心肌组织中几乎没有观察到水凝胶的片段.控释双生长因子海藻酸钠水凝胶对细胞活性无明显影响.结论 成功制备了控释双生长因子海藻酸钠水凝胶载体,具有较好的降解性能及安全性,它可以作为具有控制释放生长因子能力的生物载体材料,为后续实验提供基础.%Objective To prepare two growth factors controlled-release alginate hydrogel carrier and detect their characteristics.Methods Sodium alginate was modified through oxidization and cobalt-60 source irradiation. Two growth factors controlled-release alginate hydrogel carrier were prepared. Gel degradation behavior was monitored by measuring the dry weight loss over time and degradation curve was obtained. The myocardium of 15 rabbits was injected by needle-free injection with 100 μl two growth factors alginate hydrogel. Myocardium was received over time point and stained with hematoxylin and eosin. Gel degradation in vivo was obtained. Cytotoxicity of gels was measured by MTT assay. Results Two growth factors controlled-release alginate hydrogels exhibited a relatively rapid degradation in vitro; within 7 days, approximately 50％ of the initial gel mass was lost. Within 21 days, approximately 100％ of the initial gel mass was lost. Following injection in vivo, gels were decreased gradually. No gel fragments was found in histological sections 5 weeks after delivery. For gels, there was no significant effect on cell activity. Conclusions Two growth factors controlled-release alginate hydrogel carrier is successfully prepared with satisfied delegation and safety, which can supply following experiment.
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