丹参酮ⅡA对自发性高血压大鼠血清前胶原末端肽的影响

摘要

目的 研究丹参酮ⅡA(TSN)对于自发性高血压大鼠(SHR)血清I型前胶原羧基端肽(PⅠCP)和Ⅲ型前胶原氨基端肽(PⅢNP)水平的影响.方法 24只8周龄SHR大鼠随机分为三组:SHR对照组,丹参酮组,缬沙坦组.另有8只相同周龄Wistar大鼠为正常对照组.给予相应的药物治疗4 w后,测量鼠尾血压和心室质量,用VG染色检测心肌胶原容积分数(CVF),并用放射免疫分析法测定血清PⅠCP和PⅢ NP浓度.结果 SHR对照组血压、心室质量、心室质量指数、CVF均显著高于其他组(P＜0.01);血清PⅠCP和PⅢNP水平亦明显升高(P＜0.05).经丹参酮和缬沙坦治疗4 w后,其血压(P＜0.01)、心室质量、心室质量指数(P＜0.05)、CVF(P＜0.01)较SHR对照组有不同程度的降低;血清PⅠCP和PⅢ NP水平较SHR对照组亦有不同程度的降低(P＜0.05).结论 TSN能够抑制心肌纤维化,其机制可能与其抑制心肌胶原合成有关.%Objective To study the effects of Tanshinone on serum levels of procollagen type Ⅰ carboxy terminal peptide ( P Ⅰ CP)and procollagen type Ⅲ amino terminal peptide ( PⅢ NP) in spontaneously hypertensive rats(SHRs).Methods Twenty four male SHRs were randomly divided into positive control, Tanshinone and valsartan groups.Eight male Wistar rats were as normal control group.After 4 w intervention, blood pressure (BP) of the rat tail, weight of myocardium were measured ,the collagen volume fraction(CVF) in the myocardial tissues were calculated by VG staining and the contents of P Ⅰ CP, PⅢNP were measured with radio immunoassay.Results BP, ventricular mass, ventricular mass index and CVF of positive control group were higher than those of other groups (P ＜0.01 ).The contents of P Ⅰ CP and PⅢ NP in positive control group also were increased significantly than those in other groups (P ＜0.05 ).After 4 w intervention,BP (P ＜ 0.01 ) , ventricular mass, ventricular mass index ( P ＜ 0.05 ) and CVF ( P ＜ 0.01 ) of Tanshinone group and valsartan group were lower than those of positive control group.The contents of P Ⅰ CP and PⅢ NP in Tanshinone group and valsartan group were also decreased significantly than those in positive control groups (P ＜ 0.05).Conclusions Tanshinone could inhibit myocardial fibrosis of SHRs, which may be by suppressing the synthesis of collagen.