Objective To establish an experimental Fusaium keratitis in a guinea pig model , which is an available animal model to the clinical and basic research for fungal keratitis .Methods The animal model of Fusaium keratitis was created by corneal scratching and corneal stroma injection methods with different spore suspension concentration of Fusarium solani .According to the symptoms of infection , the results of tissue pathological and etiological examination , the optimal inoculation method and concentration were determined .Results Guinea pig models created by corneal scratching method with mild symptoms had a lower infection rate and were easily to healing .However models created by corneal stroma injection method with the severe and persistent symptoms had a higher infection rate .Meanwhile the infec-tion rate increased with the increasing spore suspension concentration .Conclusions An experimental Fusaium keratitis in a guinea pig model is successfully established by corneal stroma injection method with higher spore suspension concentration .This animal model is practical for the further study of fungal keratitis .%目的：探讨豚鼠镰刀菌性角膜炎模型的建立方法，为真菌性角膜炎的临床和基础研究提供理想的动物模型。方法采用角膜划痕法和角膜基质注射法以不同浓度茄病镰刀菌孢子悬液感染豚鼠，建立镰刀菌性角膜炎模型，观察感染症状，进行组织病理学和病原学检查，判断最适感染方式和接种浓度。结果划痕法感染角膜病变较轻，易自愈，感染率较低；基质注射法感染病变较重，且较持久，感染率较高。随着接种孢子悬液浓度的增加感染率增高。结论利用角膜基质注射法接种较高浓度的孢子悬液成功地建立了豚鼠镰刀菌性角膜炎模型，为真菌性角膜炎的进一步研究奠定了基础。
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