载脂蛋白E基因型在老年人群中的分布及与老年认知损伤的关系

摘要

目的：研究老年人中载脂蛋白（ Apo） E基因型的分布状况，分析ApoE E4基因型与老年认知损伤发生的关系。方法随机选取546例老年人，根据MoCA Version量表测评的内容进行老年认知损伤的早期筛查，抽取血液并分离白血细胞基因组DNA，用PCR技术扩增 ApoE基因多态性位点，检测其基因型。结果在老年人群中ApoE E3／3为最常见的基因型，占72．89％，而E2／2最少见，占1．28％；认知损伤的老年人中 ApoE E4等位基因频率明显高于对照组（χ2＝46．658，P＜0．001）。结论携带 ApoE E4基因的老年人群是痴呆的高危人群，需要早干预，防止或延缓痴呆的发生。%Objective To study the frequency of apolipoprotein ( Apo) E genotypes in elderly and analyze the relation between ApoE and mild cognitive impairment .Methods 546 elder cases were assessed by MoCA Version for early screening .White blood cell genomic DNAs were separated .ApoE polymorphism sites were amplified by PCR .Results The common genotypes was ApoE E 3/3 which was 72.89% and the least was E2/2 which was 1.28%.Genotypes of ApoE E4 in mild cognitive impairment elderly was higher than that in com-mon elderly (χ2=46.658,P<0.001).Conclusions The elderly with ApoE E4 are risk population for mild cognitive impairment , early in-tervention is necessary to prevent the mild cognitive impairment .