石蜡包埋子宫内膜样腺癌中雌激素硫酸转移酶和甾体硫酸酯酶基因的表达

摘要

Objective To explore the expression of estrogen sulfotransferase(EST) and steroid sulfatase (STS) in formalin-fixed paraffin-embedded endometrioid adenocarcinoma samples. Methods The RNA of EST and STS in 30 formalin-fixed paraffin-embedded endometrium samples were extracted using Roche products. Results The RNA expression of EST and STS were 0.25±0.03 and 0.08±0.02 respectively and the STS/EST was 0.11±0.08 in normal endometrium. While in endometrioid adenocarcinoma the RNA expression of EST and STS were 0.06±0.02 and 0.24±0.92 respectively and the STS/EST was 4.40±0.64. There were significant differences between these two groups. Conclusions (1) Formalin-fixed paraffin-embedded tissues could be used to study the endometrioid adenocarcinoma. (2) EST is decreased, STS and STS/EST are increased in human endometrioid adenocarcinoma. STS/EST may be related with the prognosis of the endometrioid adenocarcinoma.%目的 探讨石蜡包埋子宫内膜样腺癌组织中雌激素硫酸转移酶(estrogen sulfotransferase,EST)和甾体硫酸酯酶(steroid sulfatase,STS)的RNA表达. 方法 使用罗氏产品从30例石蜡包埋子宫内膜组织(对照组和患病组各15例)中提取EST、STS的RNA. 结果 对照组RNA的表达EST为0.25±0.03,STS为0.08±0.02,STS与EST比值为0.11±0.08;患病组RNA的表达:EST为0.06±0.02,STS为0.24±0.92,STS与EST比值4.40±0.64,患病组与对照组比较,差异有统计学意义(t分别为-8.223、7.345和11.591,均P<0.05). 结论 (1)石蜡包埋组织是子宫内膜样腺癌基础研究的材料来源;(2)在子宫内膜样腺癌中,EST下降,STS和STS与EST比值升高.