5-氮胞苷对大鼠骨髓基质细胞心钠素及β-肌球蛋白重链表达的影响

摘要

Objective To assess the effect of 5-azacytidine (5-AZ) on the expressions of atrial natriuretic peptide (ANP) and β-myosin heavy chain (β-MHC) genes in cultured rat bone marrow stromal cells (BMSCs). Methods Neonatal rat cardiomyocytes and adult rat BMSCs were isolated and cultured. BMSCs were incubated with 5-AZ and (or) myocardiocytes culture medium. RT-PCR was used to measure the expressions of cardiac ANP and β-MHC genes. Results Normally cultured rat BMSCs didn't express cardiac ANP or β-MHC. After inducing by 5-AZ, rat BMSCs expressed ANP or β-MHC. The myocardiocytes culture medium enhanced expression of β-MHC, but didn't affect the expression of ANP. Conclusions The 5-AZ induces the rat BMSCs to express either cardiac ANP or β-MHC, and the former could be enhanced by cardiomyocytes culture medium.%目的 观察5-氮胞苷(5-AZ)诱导后体外培养骨髓基质细胞(BMSCs)心钠素(ANP)、β肌球蛋白重链(β-MHC)表达的变化.方法 分离培养SD大鼠BMSCs及新生乳鼠心室肌细胞.BMSCs的诱导分化采用第8代BMSCs,于传代后第3 d分为4组:正常对照组、上清液组、5-AZ组、5-AZ+上清液组.反转录聚合酶链反应法测定心肌特异性蛋白ANP、β-MHC基因表达水平的变化.结果 正常培养及心肌细胞上清培养液诱导的BMSCs不表达心肌特异性ANP、β-MHC;5-AZ诱导后的BMSCs表达ANP、β-MHC,分别31.5±5.6、32.1±8.3和33.7±5.6、46.6±8.3.心肌细胞上清培养液增加5-AZ诱导的BMSCs中β-MHC的表达水平,而对ANP表达无影响.结论 体外培养大鼠成体BMSCs在5-AZ诱导下可表达心肌特异性ANP、β-MHC.心肌细胞上清培养液可增加β-MHC表达水平.