大鼠大脑皮层神经元缺氧后缺氧诱导因子-1α的表达

摘要

目的 探讨缺氧诱导因子-1α(HIF-1α)在缺氧后大脑皮层神经元的表达情况,为临床治疗缺血缺氧性脑血管病提供更多的实验依据.方法 制备体外培养大鼠大脑皮层神经元原代培养及缺氧的模型;免疫细胞化学鉴定大鼠大脑皮层神经元;在12h,24 h,48 h及72 h采用免疫组化方法检测正常及缺氧神经元中HIF-1α的表达情况.结果 正常对照组神经元各时间点HIF-1α阳性细胞表达较少,组内各时间点比较差异无统计学意义.缺氧组神经元在缺氧12 h时已观察到HIF-1α的弱阳性表达,随缺氧时间的延长阳性表达增强,24 h表达明显增加(光密度为0.27±0.02,F＝35.703,t＝11.795,P＜0.01),48 h达高峰,一直持续到72 h下降,两组各时间点相比差异有统计学意义.结论 神经元缺氧后HIF-1α表达上升,对缺氧后的神经元起保护作用.%Objective To investigate the expression of hypoxia-inducible factor 1α (HIF-1α)gene in rat cerebral cortex neurons under hypoxia conditions,and provide more experimental basis for clinical treatment of hypoxic brain disease.Methods The rat cerebral cortical neurons in primary culture and hypoxia model were prepared and identified by immunocytochemistry analysis.The expression of HIF-1α in normal and hypoxic neurons was detected at 4 time points (12 h,24 h,48 h and 72 h) by immunohistochemical analysis.Results The expression of HIF-1α positive cells in normal control (NC) group was less at each time point,and it showed no statistically significant within groups.The weak expression of HIF-1α was found at 12 h in hypoxic group,and the expression augmented along with the time extended,it increased obviously at 24 h,reached the peak at 48 h(IOD＝0.27±0.02,F＝35.703,t＝11.795,P＜0.01),and declined until 72 h,it showed statistical significance between two groups at each time point.Conclusions The expression of HIF-1α is increased after hypoxia.HIF-1α has a protective effect on neurons after hypoxia.