Objective To explore the relationship of endothelial lipase (EL) 584C/T gene polymorphism with unstable angina pectoris (UAP) and EL level in middle-aged and elderly patients in Changzhou.Methods The EL584C/T genotypes were detected in 158 UAP and 278 health controls by PCR-RFLP methods and the EL level by ELISA assay.Results (1)The frequencies of the CC,CT and TT genotypes were 57.6％ (91 cases),36.1％ (57 cases) and 6.3％ (10 cases) in the UAP group versus 56.8％ (158 cases),39.9％ (111 cases) and 3.2％ (9 cases) in control group respectively (x2 =2.61,P=0.271).No significant differences in T versus C allele frequencies were found between UAP and control groups (x2 =0.16,P=0.742).In logistic regression,there was no significant relationship between the EL 584C/T gene polymorphism and UAP recurrence after adjusting for traditional UAP risk factors.(2)The serum EL level was higher in UAP group (24.7±18.6) μg/Lthan in control group (19.3±9.5) μg/L (t=2.26,P=0.041).(3)There was no significant difference in the serum EL level among different EL584C/T genotypes.Conclusions No significant relationships of EL584C/T gene polymorphism with UAP and EL level are found in patients in Changzhou region.%目的 探讨中国常州地区不稳定型心绞痛(unstable angina pectoris,UAP)患者内皮脂肪酶(endothelial lipase,EL)基因584C/T多态性与EL血清水平的相关性. 方法 采用聚合酶链反应-限制性片断长度多态性分析(polymerase chain reaction restriction fragment length polymorphism,PCR-RFLP)检测158例UAP组和278例对照组EL584C/T基因多态性;随机选取48例UAP患者和70例健康对照者,采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)测定血清EL水平. 结果 UAP组CC、CT、TT基因型频率分别为91例(57.6％)、57例(36.1％)、10例(6.3％),对照组CC、CT、TT基因型频率分别为158例(56.8％)、111例(39.9％)、9例(3.2％),两组基因型频率分布差异无统计学意义(x2 =2.61,P=0.271);两组T等位基因频率分别为79例(22.6％)、129例(23.2％),差异无统计学意义(x2 =0.16,P=0.742).经Logistic回归分析,在校正传统UAP的危险因素后,结果仍显示EL584C/T基因多态性与UAP的发病无关.UAP组血清EL水平为(24.7±18.6) μg/L,高于对照组(19.3±9.5) μg/L(t=2.26,P=0.041).EL584C/T不同基因型间血清EL水平差异无统计学意义. 结论 EL584C/T基因多态性与常州地区人群UAP发病无关,且该多态性与其血清水平无相关性.
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