Objective To study the RBC "piping phenomenon" in hematoma due to intracerebral hemorrhage in rats .Methods Sixty SD male rats were randomly divided into experimental group (n=30) and control group(n=30) .Each group was further divided into 1 h group ,6 h group ,24 h group ,3 d group ,7 d group(5 ineach group).A rat model of autologous cerebral artery hemorrhage was established with FITC probe-labeled RBC .Brain and neck deep lymph node tissues were cut into sections which were stained with HE and observed under fluorescence microscope . The distribution features of RBC were analyzed .Results The FITC-RBC and free-FITC were distributed in experimental group ,bilateral cerebral hemisphere ,brain stem Virchow-Robin spaces , and bilateral neck deep lymph nodes 1 h after hematoma formation .Yellow or yellow-green flores-cent granular phagocytes were observed ,the FITC-RBC were gradually disappeared and the number of free-FITC began to decrease 6 h after hematoma formation . The number of yellow or yellow-green florescent granular phagocytes began to increase and yellow florescent granules could be found in lymph node germinal center . No such phenomina were observed in control group .Conclusion The spread of RBC "piping phenomenon" is limited while the free-RBC "piping phenomenon" is extensive in hematoma due to intracerebral hemorrhage .The RBC "piping phenomenon" is one of the absorption ways of RBC in hematoma .%目的 探索脑出血后血肿内红细胞通过"管涌现象"的分布特征.方法 SD雄性大鼠60只,随机分为实验组30只和对照组30只,每组分为5个不同观察时间点:1 h、6 h、24 h、3 d、7 d,每个时间点5只.采用异硫氰酸荧光素(FITC)标记血液中红细胞,建立大鼠自体动脉血脑出血模型,分别于不同时间点进行大鼠脑组织、颈深淋巴结组织病理切片,荧光显微镜下观察以及HE染色后显微镜下观察,对比分析红细胞的分布特征.结果 实验组血肿形成1 h后,病灶以及血肿周围可见到FITC-RBC和游离FITC分布,双侧大脑半球和脑干的维乔-罗宾间隙内也有游离FITC分布,双侧颈深淋巴结内均可见FITC-RBC和游离FITC分布.6 h后血肿内开始出现含有黄色或黄绿色荧光颗粒的吞噬细胞,同时FITC-RBC和游离FITC逐渐减少.随时间延长,含有黄色荧光颗粒的吞噬细胞在各脑区内逐渐增多,淋巴结生发中心内可见成团分布的黄色荧光颗粒.对照组未见以上变化.结论 血肿内红细胞通过"管涌现象"在脑出血后扩散范围比较局限,游离红细胞成分扩散范围比较广泛;"管涌现象"是血肿内有红细胞吸收途径之一.
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