基于载脂蛋白Eε4分型的健康老年人静息态全脑功能连接强度特征研究

摘要

Objective To study the mechaniams of apoEε4 allele gene underlying the topology structure of brain function network by analyzing the characteristics of resting state functional connectivity strength (FCS) in healthy elderly people based on apoEε4 allele gene typing.Methods The demographic data,MRI data and apoEε4 data of 44 healthy elderly people were collected from the Alzheimer's Disease Neuroimaging Initiative Database.The 44 healthy elderly people were divided into apoEε4 group (n=14) and non-apoEε4 group (n=30) according to whether they carried apoEe4 allele gene.They underwent resting state functional MRI (rs-fMRI) scanning and neuropsychological assessment.The whole brain long-range FCS and short-range FCS were calculated after the rs-fMRI data were preprocessed.The difference between the two groups was compared.Results The long-range FCS of the right insular lobe was significantly lower in apoEε4 group than in non-apoEε4 group in resting state (the cluster vlume was 183 voxels,14656.88±3762.93 vs 20905.82-±-10245.85,P＜0.05),the short-range FCS of the left and right insular lobes and the right hippocampus/fusiform ratio were also significantly lower in apoEε4 group than in nonapoEε4 group in resting state (the cluster volume was 91,232 and 75 voxels,P＜0.05).Conclusion Healthy elderly apoEε4 allele gene carriers do not show cognitive impairment,but their FCS is different from that of non-apoEε4 allele gene carriers,which may be due to the potential neurobiological effect of apoEε4 allele.%目的 分析载脂蛋白E (apoE)ε4等位基因分型的健康老年人静息态全脑功能连接强度(FCS)特征,探讨apoEε4基因型调控认知正常老年人的脑功能网络拓扑组织结构的机制.方法 入选的44例健康老年人的人口统计学资料、MRI数据、apoEε4信息均来自美国公开数据库.根据是否携带apoEε4将入选者分为apoEε4组14例和非apoEε4组30例.进行静息态功能MRI(rs-fMRI)扫描和神经心理学评估,采集rs-fMRI数据进行预处理,计算全脑长程和短程FCS值,比较2组差异.结果 静息状态下,与非apoeEε4组比较,apoEε4组右侧岛叶长程FCS值减低(簇大小为183个体素,14656.88±3762.93 vs 20905.82±10245.85,P＜0.05),左侧岛叶、右侧岛叶及右侧海马/梭状回短程FCS值减低(簇大小分别为91、232和75个体素,P＜0.05).结论 携带apoEε4的健康老年人可不表现认知功能的损害,但其FCS值与非携带者存在差异,这可能是apoEε4基因潜在的神经生物学效应.