慢性丙型肝炎患者血清趋化因子CXCL9和CXCL11的检测及其临床意义

摘要

目的 探讨慢性丙型肝炎患者血清趋化因子CXCL9和CXCL11的变化规律及其临床意义.方法 用酶联免疫吸附法( ELISA)检测血清CXCL9和CXCL11的浓度；荧光定量反转录聚合酶链反应法(RT-PCR)检测HCV RNA病毒载量；INNO-LiPA线性探针杂交法检测HCV基因分型；全自动生化分析仪检测肝功能.结果 对照组(n=26)和慢性丙型肝炎组(n=48)血清CXCL9的浓度分别为( 596.8±238.4) pg/mL和(2457.8±1650.7) pg/mL,血清CXCL11的浓度分别为(106.8±76.95) pg/mL和(307.5±259.4) pg/mL,差异均具有统计学意义(P＜0.05).在慢性丙型肝炎组内,CXCL9和CXCL11的水平在不同HCV 1b基因型组和2a基因型组之间无显著差异.CXCL9和CXCL11浓度与血清ALT水平无相关性(CXCL9:r=-0.119,P=0.397；CXCL11:r=0.219,P=0.138),与HCV RNA载量也无相关性(CXCL9:r=0.253,P=0.212；CXCL11:r=0.105,P=0.451).结论 CXCL9和CXCL11可能参与了慢性丙型肝炎感染导致的肝脏免疫损伤过程,ALT和HCV RNA与CXCL9和CXCL11浓度变化无明显相关性.%Objective To investigate the changes and clinical significances of serum chemokine CXCL9 and CX-CL11 in patients with chronic hepatitis C. Methods The levels of CXCL9 and CXCL11 in the serum were measured by enzyme linked immunosorbent assay (ELISA) ; HCV RNA was detected by real-time reverse transcriptional-polymerase chain reaction (RT-PCR) ; Genotyping of HCV was performed using a second generation INNO-LiPA line probe assay; Liver function was assayed by automatic biochemistry analyzer. Results Concentrations of serum CXCL9 in normal controls (ra =26) and chronic hepatitis C (n = 48) were (596. 8 ±238.4) pg/mL and (2457. 8 ± 1650. 7) pg/mL, respectively. Concentrations of serum CXCL11 in normal controls and chronic hepatitis C were (106.8 ±76.95) pg/mL and (307.5 ±259.4) pg/mL, respectively. There were significant differences between the two groups for both CXCL9 and CXCL11 (P<0.05). The levels of the two chemokines in HCV genotype lb and 2a revealed no remarkable differences. Furthermore, there was no correlation between concentrations of the two chemokines and ALT levels, so as to the associations of CXCL9/11 and HCV RNA. Conclusion CXCL9 and CXCL11 may take part in the immunologic injury in the liver induced by HCV. The changes of serum CXCL9 and CXCL11 are not evidently related with either ALT or HCV RNA.