目的 研究骨桥蛋白(OPN)与环氧化酶-2(COX-2)在结肠癌组织中的表达水平,并探讨两者与结肠癌转移浸润的关系.方法 应用免疫组化法检测60例结肠癌组织和16例癌旁正常组织中OPN与 COX-2的表达,并用SPSS 16.0统计软件分析其表达水平与临床病理特征的关系及二者的表达相关性.结果 OPN在结肠癌组织中表达的阳性率(70.O％)高于癌旁正常结肠组织(18.8％),差异具有统计学意义(P＜0.01);COX-2在结肠癌组织中表达的阳性率(75.O％)高于癌旁正常结肠组织(37.5％),差异具有统计学意义(P＜0.01).OPN和COX-2蛋白的表达与患者的性别、年龄、肿瘤大小无关,与肿瘤分期、淋巴结转移有关(P＜0.05),OPN与COX-2蛋白在结肠癌组织中表达呈正相关.结论 OPN和COX-2在结肠癌的发生发展中起重要作用,联合检测可作为判断结肠癌恶性程度和预后的指标.%Objective To investigate the expression of osteopontin (OPN) and Cycloxygenase-2 ( GOX-2 ) in colon cancer, and to analyze the correlation of OPN and COX-2 with invasion and metastasis of colon cancer. Methods The immunohistochemistry was used to detect the expression of OPN and COX-2 in 60 cases of colon cancer and 16 cases of adjacent normal tissues. The relationship between expressions of proteins and clinicopathologic factors was evaluated. Results The expression of OPN was 70.0% in cancer tissues. It was significantly higher than that in the adjacent normal tissues (P<0.01). The expression of COX-2 was 75.0% in cancer tissues. It was significantly higher than that in the adjacent normal tissues ( P <0. 01 ). The expression of OPN and COX-2 were associated with tumor stage and lymph node metastasis (P <0.05) , but not with age, gender and tumor size. The expression of OPN was positively correlated to COX-2. Conclusion Bolh OPN and COX-2 play important roles in the development of colon cancer, and the joint test of them could be used as indicators of malignancy and prognosis for colon cancer.
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