神经酸对小肠隐窝细胞IEC-6紫杉醇损伤的保护机制研究

摘要

Objective To investigate the effect of nervonic acid ( NA) on IEC-6 cell induced by paclitaxel ( PTX) , including mechanism and vitality. Methods IEC-6 cell treated with PTX to detective PTX IC50 . IEC-6 cells' relative proliferative rates of NA in different levels (5 μmol/L, 10 μmol/L, 20 μmol/L) were detected by MTS assay, the expression levels of A-FABP, p38, Bcl-2, Bax, Bcl-2/Bax, CytC and cl-Caspase-3 were detected by Western blotting, and apoptotic cells of IEC-6 were detected by TUNEL. Results PTX could encourage normal cells apoptosis. NA was characterized with biological functions such as maintenance of cell shape and vitality. TUNEL showed that PTX could promote cells apoptosis, and NA had a protective effect on IEC-6 apoptosis induced by PTX. The expressions of A-FABP, p-p38, Bax, CytC, cl-Caspase-3 were reduced and Bcl-2, Bcl-2/Bax expression were increased in NA group compared with PTX group ( P <0 . 01 ) . There was no significant difference of p38 in two groups ( P >0 . 05 ) . Conclusion NA can reduce paclitaxel-induced apoptosis of IEC-6 cells by regulating p38 pathway and Bcl-2/Bax.%目的 探讨神经酸(nervonic acid,NA)对紫杉醇损伤的小肠隐窝细胞的保护机制.方法 采用紫杉醇处理IEC-6细胞,检测紫杉醇IC50浓度.MTS法检测不同浓度NA(5μmol/L、10μmol/L、20μmol/L)对紫杉醇(IC50浓度)干预后的IEC-6细胞的相对增殖率.原位缺口末端检测法(TUNEL法)检测DNA断裂情况.蛋白质印迹法检测相关蛋白表达.结果 紫杉醇促进正常IEC-6细胞凋亡.NA对维持细胞形态、保持细胞活力有一定作用.TUNEL法显示,与空白对照相比较,紫杉醇组细胞凋亡明显增加;与紫杉醇组细胞比较,NA组细胞凋亡明显减少.与紫杉醇组比较,NA组A-FABP、p-p38、Bax、CytC、cl-Caspase-3降低(P<0.01),Bcl-2、Bcl-2/Bax升高(P<0.01).p38无明显变化(P>0.05).结论 NA可通过调节p38通路、Bcl-2/Bax等减轻紫杉醇诱导的小肠隐窝细胞的凋亡损伤.