大鼠骨髓间充质干细胞分化成肝细胞过程中差异基因的功能研究

摘要

目的 探寻大鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)分化成肝细胞过程中的核心调控机制.方法 利用芯片检测结果,筛选大鼠骨髓MSCs诱导分化成肝细胞样细胞(hepatocytelike cells,HLCs)、正常肝细胞(normal liver cells,NLCs)动态发展过程中差异表达的基因,利用基因的表达变化进行表达趋势聚类,筛选出显著性的表达趋势.集中分析具有相同趋势的共表达基因,构建基因的共表达调控网络,从中筛选在动态变化过程中的核心基因.结果 从MSCs分化成NLCs的动态过程中,通过筛选得到4 938个差异表达基因,Fas、G6pc、IL1b、S100a1、Ndufa7等基因为关键基因.结论 MSCs最终分化成NLCs的过程中,与细胞周期调控、增殖、分化相关基因及糖、脂肪酸、胆固醇的代谢、解毒相关基因表达密切相关.%Objective To explore the possible mechanism of the central regulatory mechanism from rat bone marrow mesenchymal stem cells (BMSCs) differentiated into hepatocytes.Methods Differentially expressed genes in the dynamic development process of BMSCs differentiated into hepatocyte like cells (ALCs) and normal liver cells (NLCs) were screened.Expression trends by gene expression changes were clustered and a significant trend of expression was screened out.The co-expression genes with the same trend were analyzed,co-expression regulatory network of genes was constructed.Core genes in dynamic change process were screened.Results In the dynamic process from BMSCs differentiated into NLCs,4 938 differentially expressed genes were screened out.Fas,G6pc,IL1b,S100a1,Ndufa7 and other genes were the key genes.Conclusion In the process of BMSCs eventually differentiated into NLCs,BMSCs is closely related to cell cycle regulation,proliferation,differentiation and related genes,sugar,fatty acids,cholesterol metabolism,detoxification related gene expression.