法医STR分型电泳凝胶稳定参数的探讨

摘要

目的 探讨对法医STR基因分型检测使用的毛细管电泳筛分介质进行评价的参数,为电泳凝胶验证评价提供参考指标.方法 采用ABI 3130xl遗传分析仪,以3130 POP-4TM凝胶为筛分介质,以分子量内标GS500LIZ和Typer500为样本,多批次进行毛细管电泳；对原始数据进行分组统计,得到单碱基对相对迁移时间及其相对标准偏差(RSD).结果 分子量内标GS500LIZ和Typer500在POP-4TM凝胶中电泳,单碱基对相对迁移时间的统计结果表现趋势相似,即随着DNA片段增大,单碱基对相对迁移时间的均值减小,标准差及相对标准偏差增大.组间方差分析结果符合方差齐性,P ＜0.01,组间多重比较,P＜0.000 28(Bonferron校正).结论 单碱基对相对迁移时间的相对标准偏差(RSD)是毛细管电泳重现性的重要参数,可为凝胶研发和验证提供参考性指标.%Objective To explore the stability parameters of polymers for forensic DNA typing, and provide references for evaluation of electrophoresis gel. Methods On the ABI 3130x1 genetic analyzer with POP-4? As the sieving metrix, two different internal size standard, GS500LIZ and Typer500, were run repeatedly as samples. By collecting the raw data and grouping them, the relatively migrating time and its relatively standard deviation (RSD) in lbp of each internal size standard were achieved. Results The relatively migrating time of 1 bp of each internal size standard exhibited the same tradition: as the size of DNA fragments increased, the average of the relatively migrating time decreased, and the RSD increased. Analysis of variance between groups met equal variances, P <0. 01, and multiple comparisons between groups,P < 0.000 28(Bonferron revised). Conclusion The RSD of the relatively migrating time in lbp is a crucial parameter for the reproducibility on capillary electrophoresis system, and can be served as a parameter in exploration and validation of polymers.