Nucleotide sequences of mitochondrial DNA 12S rRNA partial sequence were amplified in six wild populations of fat greenling Hexagrammos otakii using PCR techniques. A total of 314 variable sites were detected among the aligned sequences of 582 bp with mutation rate of 53.95%. Sixteen haplotypes were identified from 34 individuals according to the determined sequences, and the six populations had haplotype diversity of 0.40 ~ 1.00, nucleotide diversity of 0.00071 ~ 0.10441 and Fu's Fs of 1.19782 in neutral-ity tests(P < 0.01). AMOVA analysis demonstrated that: there were Fst = 0.88326(P < 0.01), variance of 88.33% among six popula-tions and variance of 11.67% within the six populations. The NJ and MP molecular phylogenetic trees constructed by the distances a-mong different individuals revealed that the phylogenetic trees were all divided into two branches, one in Donggang and Lvshun popu-lations and the others in the other populations.%本文利用PCR技术扩增了6个野生群体大泷六线鱼线粒体12S rRNA基因的部分序列.在获得的582bp碱基序列中,共检测到314个变异位点,占总位点数的53.95%.34尾个体共定义了16种单倍型,6个群体的单倍型多样性指数为0.40~1.00,核苷酸多样性指数为0.00071~0.10441.中性检验Fu's Fs值为1.19782(P<0.01).分子变异分析表明:Fst=0.88326(P<0.01),群体间和群体内变异分别为88.33%和11.67%.不同个体间的遗传距离构建的NJ和MP系统树表明,东港和旅顺群体亲缘关系较近,构成一个分支,其他群体构成另一分支.
展开▼
