Toll样受体2和4在输卵管慢性炎症组织中的表达

摘要

目的:探讨Toll样受体2和4(TLR2、TLR4)在输卵管组织中的表达及与输卵管慢性炎症病变的关系.方法:选择201 1年1～3月在嘉兴市妇幼保健院手术切除输卵管的病例45例,经过患者知情同意后开展本研究.将临床获得的标本分3组:输卵管正常组(10例)、轻度病变组(18例)和重度病变组(17例).用免疫组化和定量PCR检测TLR2、TLR4在输卵管组织中的表达.结果:TLR2和TLR4在各组输卵管上皮细胞的胞浆和胞膜均有表达.在轻度病变组中,TLR2蛋白及mRNA表达均高于正常组(P均＜0.05)和重度病变组(P均＜0.01)；TLR4蛋白表达高于重度病变组(P＜0.05),与正常组表达无差异,TLR4 mRNA表达高于正常组和重度病变组(P均＜0.01).TLR2、TLR4、TLR2mRNA和TLR4mRNA每种物质在重度病变组表达与正常组相比,差异均无统计学意义(P均＞0.05).结论:输卵管组织表达TLR2、TLR4,并在慢性炎症轻度病变时表达上调,随着炎症加重致输卵管发生严重病变时表达下降,推测其可能参与输卵管炎症病变的发生发展.%Objective:To explore the relationship between expressions of Toll-like receptor (TLR) 2 and 4 in oviduct and chronic salpingitis.Methods:Forty-five women experiencing salpingectomy between Jan and March,2011 in Maternity & Child Health Hospital of Jiaxing were divided into 3 groups:normal oviduct (group A,n =10),mild chronic salpingitis (group B,n =18) and severe chronic salpingitis (group C,n =17).Expressions of TLR2 and TLR4 proteins and mRNA in the oviduct were evaluated by immunocytochemistry and real-time PCR,respectively.Results:TLR2 and TLR4 proteins were expressed on the epithelium cells of cytomembrane and in cytoplasm.Expressions of TLR2 protein and TLR2 mRNA in group B were significantly higher than those of group A (P all ＜ 0.05) and group C (P all ＜ 0.01).In group B,the expression of TLR4 protein was significantly higher than that of group C (P ＜ 0.05),and TLR4 mRNA level was significantly higher than those in group A and group C (P all ＜ 0.01).Moreover,there were no significant differences in expressions of TLR2 and TLR4 proteins as well as their mRNA between group A and group C.Conclusion:TLR2 and TLR4 are expressed in oviduct and maybe involved in the pathogenesis and development of chronic salpingitis.