小鼠改良微创主动脉弓缩窄模型与经典模型的比较

摘要

目的：比较改良小鼠主动脉弓缩窄（ TAC）模型与经典模型的优缺点并详细介绍改良方式。方法将C57BL／6小鼠随机分为四组：经典Sham组（ Sham－C）、经典TAC组（ TAC－C）、改良Sham组（ Sham－N）和改良TAC组（ TAC－N），比较手术时间、术中及术后4周小鼠生存率等指标。术后4周超声心动图检测心功能，心体比、心胫骨比、心脏HE染色用于反映心肌肥大程度。结果 TAC－C组手术时间为（24．88±2．99）min，而TAC－N组只需（6．90±0．63）min；TAC－C组术中死亡1例，术后4周死亡2例，生存率80％，而TAC－N组术中死亡1例，术后无死亡，生存率为93．3％；超声检查、大体指标及HE染色均显示两种模型术后4周心肌肥厚程度无明显差异。结论小鼠改良微创TAC术构建心肌肥厚模型，安全、耗时短、重复性好、成功率高。%Objective To comprehensively and technically introduce a novel transverse aortic constriction( TAC) surgery mod⁃el in mice and compare this relatively less invasive surgical modelto the classic TAC model. Methods C57BL/6 mice were divided in⁃to four groups:Sham-Classic;TAC-Classic;Sham-Novel;TAC-Novel. Mean operating time and overall survival rate during operating time and at week-4 were measured. Echocardiography was used to determine cardiac function. HW/BW, HW/TL and HE staining were used to measure the degree of cardiac hypertrophy. Results The average operating time using classic TAC surgery is 24.88±2.99 min, while TAC-N requires only 6.90±0.63 min ( P<0.01). The survival rate is 80% (TAC-C) vs 93.3% (TAC-N). No significant difference was shown in echocardiograph, HE staining, HW/BW and HW/TL between these two models. Conclusion This new ap⁃proach is more efficient and safer for mice while not compromising the extent of cardiac hypertrophy.