丹参酮ⅡA对成骨细胞MC3T3-E1增殖、凋亡以及细胞周期分布的影响

摘要

目的 观察丹参酮ⅡA对成骨细胞MC3T3-E1增殖、凋亡以及细胞周期分布的影响.方法 将成骨细胞MC3T3-E1分为4组,分别为空白对照组、低浓度组(5×10-3 mg/L)、中浓度组(5×10-2 mg/L)与高浓度组(5×10-1 mg/L).空白对照组不加任何药物,只使用常规细胞培养液;低浓度组、中浓度组、高浓度组分别加入相应浓度比例的丹参酮ⅡA进行细胞培养,噻唑蓝(MTT)法检测甲状腺癌细胞增殖能力,流式细胞仪检测细胞周期及凋亡.结果 低、中、高浓度组在不同时间段成骨细胞MC3T3-E1的增殖率显著高于空白对照组,高浓度组在不同时间段成骨细胞MC3T3-E1的增殖率显著高于低浓度组(F=2.140、2.130、3.120,P＜0.05).低、中、高浓度组在不同时间段成骨细胞MC3T3-E1的凋亡率显著低于空白对照组,高浓度组在不同时间段成骨细胞MC3T3-E1的凋亡率显著低于低浓度组(F=2.390、2.990、3.020,P＜0.05).低、中、高浓度组在不同时间段的处于G0/G1期的细胞比例显著高于空白对照组,处于S期与G2/M期的细胞比例显著低于空白对照组,高浓度组在不同时间段的处于G0/G1期的细胞比例显著高于低浓度组和中浓度组,且处于S期与G2/M期的细胞比例显著低于低浓度组和中浓度组(F=2.310、2.700、2.340、2.170、4.920、4.420、2.840、2.120、4.180,P＜0.05).结论 丹参酮ⅡA可以促进成骨细胞MC3T3-E1的增殖,抑制成骨细胞MC3T3-E1的凋亡,可明显改善成骨细胞MC3T3-E1的周期分布.%Objective Objective to investigate the effects of tanshinone Ⅱ A on proliferation,apoptosis and cell cycle distribution of osteoblasts MC3T3-E1.Methods The osteoblasts MC3T3-E1 were divided into four groups:blank control group,low concentration group (5 × 10-3 mg/L),medium concentration group (5 × 10-2 mg/L) and high concentration group (5 × 10-1 mg/L).The control group was treated with routine cell culture medium without any drugs,while the low concentration group,the middle concentration group and the high concentration group were treated with tanshinone Ⅱ A to culture the cells,methyl thiazol tetrazolium (MTT) assay was used to detect the proliferation of thyroid cancer cells,and flow cytometry was used to detect the cell cycle and apoptosis.Results The proliferation rate of osteoblasts MC3T3-E1 in low,middle and high concentration group was significantly higher than that in blank control group at different time points,and that in high concentration group was significantly higher than that in low concentration group at different time points (F =2.140,2.130,3.120,P ＜ 0.05).The apoptosis rate of osteoblasts MC3T3-E1 in low,middle and high concentration groups was significantly lower than that in blank control group at different time points,and that in high concentration group was significantly lower than that in low concentration group at different time points (F =2.390,2.990,3.020,P ＜0.05).The proportion of cells in G0/G1 phase in low,middle and high concentration group was significantly higher than that in blank control group at different time,and the proportion of cells in S phase and G2/M phase was significantly lower than that in blank control group,andthe proportion of cells in G2/M phase was significantly lower than that in low concentration group and medium concentration group (F =2.310,2.700,2.340,2.170,4.920,4.420,2.840,2.120,4.180,P ＜0.05).Conclusion Tanshinone Ⅱ A can promote the proliferation of osteoblasts MC3T3-E1,inhibit the apoptosis of osteoblasts MC3T3-E1,and significantly improve the cycle distribution of osteoblasts MC3T3-E1.