Src家族酪氨酸激酶Fyn在人食管鳞状细胞癌中的表达

摘要

目的 探讨食管鳞状细胞癌组织中Fyn的表达水平及其意义.方法 收集13例食管鳞状细胞癌组织(ESCC)和10例食管正常黏膜上皮组织(UNR),采用免疫组织化学法和免疫印迹法分析食管鳞状细胞癌组织及食管正常黏膜组织Fyn蛋白表达水平的变化.结果 免疫组织化学染色显示食管鳞状细胞癌组织中Fyn蛋白表达水平(9.68±2.31)高于食管正常黏膜组织中染色指数(3.21±1.25),差异有统计学意义(P<0.01).免疫印迹结果亦显示食管鳞癌组织中Fyn蛋白表达强于食管正常黏膜组织.结论 Fyn蛋白在食管鳞状细胞癌组织中高表达,提示Fyn基因在食管鳞状细胞癌的发生发展过程中可能起重要作用.%Objective To determine whether Fyn expression was altered in esophageal squamous cell carcinoma (ESCC) compared with unremarkable epithelium (UNR). Methods The Fyn expression was detected in ESCC and compared to the UNR by immunohistachemical staining and Western blotting. Results By immunohistochemical staining Fyn protein expression levels were increased in ESCC (9. 68± 2. 31 ) compared to the esophageal UNR (3.21 ± 1.25 ) ,P <0. 01. The similar results were achieved by Western blotting. Conclusion Fyn may act as an important molecule in the esophageal SCC regulation.