背景 角膜接触镜的佩戴可能会增加角膜感染的机会,一些研究认为角膜接触镜材料的透氧性与角膜感染的发生有关. 目的 比较用于制作硬性透氧性角膜接触镜(RGP-CL)的材料,如氟硅丙烯酸酯A(XO)、氟硅丙烯酸酯B(EO)和聚甲基丙烯酸甲酯(PMMA)表面对金黄色葡萄球菌、表皮葡萄球菌和铜绿假单胞杆菌的黏附情况. 方法 分别将3种RGP-CL镜片材料置于3种细菌悬液中培养24 h,采用MTT比色法、旋涡震荡菌落计数法和扫描电子显微镜观察并比较3种材料表面黏附细菌的能力. 结果 MTT比色法结果显示,XO制镜材料对金黄色葡萄球菌的黏附能力(A值)明显低于EO材料和PMMA材料,差异均有统计学意义(q=7.379、8.207,P＜0.01),但EO材料与PMMA材料对金黄色葡萄球菌黏附能力的比较差异无统计学意义(q=0.828,P＞0.05)；XO材料及EO材料表面表皮葡萄球菌的黏附能力低于PMMA材料,差异均有统计学意义(q=14.000、12.800,P＜0.01),而XO材料与EO材料间表皮葡萄球菌黏附能力的比较差异无统计学意义(q=1.200,P＞0.05)；3种材料对铜绿假单胞杆菌的黏附能力(A值)差异无统计学意义(F=2.155,P=0.138).旋涡震荡菌落计数法结果显示,XO、EO、PMMA材料表面金黄色葡萄球菌的黏附数量分别为(37.9±1.5)×106、(49.9±2.2) ×106、(67.4±1.6)×106个,差异有统计学意义(F=206.240,P=0.000),EO材料、PMMA材料表面金黄色葡萄球菌菌落计数明显高于XO材料,差异均有统计学意义(P＜0.01).XO、EO、PMMA材料表面表皮葡萄球菌的黏附数量分别为(7.9±1.3)×106、(10.5±1.5) ×106、(11.2±1.2)×106个,XO材料表面表皮葡萄球菌的黏附数量明显低于PMMA材料,差异有统计学意义(q=5.060,P＜0.05),XO材料与EO材料、EO材料与PMMA材料之间表皮葡萄球菌黏附数量的差异均无统计学意义(q=3.290、1.770,P＞0.05).3种材料表面铜绿假单胞杆菌的黏附数量差异无统计学意义(F=0.232,P=0.799).扫描电子显微镜观察显示,XO材料和EO材料表面金黄色葡萄球菌和表皮葡萄球菌的黏附呈散在性分布,而PMMA材料表面这两种细菌黏附形成“网状”外观,3种镜片材料表面铜绿假单胞杆菌形态无明显差别. 结论 PMMA材料表面细菌的黏附能力强于XO材料和EO材料,RGP-CL的透氧性对镜片表面细菌黏附的能力无明显影响.%Background Wearing contaclenincreasethe risk of infection of the cornea.Some studieshowed the gas-permeability of materialused foconstructing corneal contaclenione of the contributing factorrelated to corneal health.Objective Thistudy wato observe the in vitro adherence ability of differenbacterito rigid gas-permeable contaclense(RGP-CL) made with varioumaterials.MethodContaclensemade with hexafocon,enflufocon opolymethyl methacrylate (PMMA) were placed into Staphylococcuaureus,Staphylococcuepidermidis,oPseudomonaaeruginosbacterial suspension(0.5 MCF) fo24 hours.The strength of bacterial adherence watested and studied by the methyl thiazolyl tetrazolium (MTT) colorimetrimethod based on absorbance (value),and the vortex method waused to calculate the colony forming units.The bactericlump formation waexamined with scanning electron microscope (SEM).ResultMTcolorimetrimethod showed thathe adherence ability of Staphylococcuaureuto hexafocon (value) wasignificantly lowethan thato enflufocon and PMMA,respectively (q=7.379,8.207,P＜0.01),buno significandifference wafound in the adherence ability of Staphylococcuaureubetween enflufocon and PMM(q =0.828,P＞0.05).The adherence ability of Staphylococcuepidermidito XO and enflufocon walowethan thato PMM(q =14.000,12.800,P＜0.01),buno significandifference wafound between the adherence of Staphylococcuepidermidito hexafocon and enflufocon material (q =1.200,P＞0.05).There wano significandifference in the adherence ability of Pseudomonaaeruginosto all three material(F=2.155,P=0.138).The vortex method presented the colony forming unitof Staphylococcuaureuto hexafocon,enflufocon and PMMwith (37.9± 1.5)×106,(49.9±2.2)×106 and (67.4± 1.6)×106,respectively,with significandifference among them (F =206.240,P＜0.01),showing the lowesvalue in hexafocon,the highesvalue in PMMand middle value in enflufocon (q=11.650,28.640,16.990,P＜0.01),Moreover,colony forming uniof Staphylococcuepidermidito hexafocon,enflufocon and PMMwa(7.9 ± 1.3) × 106,(10.5 ± 1.5) × 106,(11.2 ±1.2) × 106,respectively.And thaof hexafocon walowethan one of the PMMmaterial (q =5.060,P＜0.05).No significandifference wafound between hexafocon and enflufocon nobetween hexafocon and PMM(q =3.290,1.770,P＞0.05).In addition,the resultthacorresponded to the vortex method were seen in the MTcolorimetriassay (F =0.232,P =0.799).SEM examination showed dispersed population of Staphylococcuaureuand Staphylococcuepidermidion the surfaceof hexafocon and enflufocon;while much more Staphylococcuaureuand Staphylococcuepidermidiadhered on the surface of PMMA,forming net-like appearance.Conversely,high numbeof Pseudomonaaeruginoswaseen on the surface of all three materials,withounoticeable differencein the bacterial shape and quantity on each of the material.ConclusionThe adherence ability of bacterito PMMistrongethan thaof hexafocon and enflufocon,and gas-permeable material of RGP-CL doenoimpacthe adherence ability of bacteria.
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