Background High myopia is seriously harmful to visual function.To explore the mechanism of high myopia is significant for the prevention and treatment.Complement proteins C1q and C3 have emerged as critical mediators of synaptic refinement and plasticity,however,their effects in retina on myopia remain elusive.Objective This study was to investigate the expressions of complement factors C1q and C3 in the retina with negative lensdefocused myopia in guinea pigs.Methods The use and care of animals complied with the application of Peking Union Medical College Hospital Iaboratory Animal Welfare Ethics Committee.Twelve 3-day-old pigmented guinea pigs were assigned randomly to the lens-defocused group and the normal control group.The left eyes were covered by -10 D PMMA lens for 4 weeks as the defocused eye group,and the right eyes were covered using 0 D PMMA lens in the same way as the fellow control eye group.The right eyes of the normal guinea pigs were used as the normal control eye group.The refractive diopter of guinea pigs was examined by retinoscopy.The animals were sacrificed at the fourth week and the expressions of complement C1q and C3 in the retinas of guinea pigs were detected by Western blot.Results The diptors were (-1.21±0.71)D,(+2.46±0.75)D and (+1.75±0.50)Din the defocused eye group,normal eye group and the fellow eye group at the fourth week,showing a significant difference among the groups (F=51.55,P=0.69),and diopters were insignificantly different between the normal eye group and fellow eye group (q =2.62,P=0.08).However,the diopters of the defocused eye group were significantly higher than those of the fellow eye group (q =10.92,P＜0.01).The expressions of C1q and C3 proteins in the retinas were significantly different among the defocused eye group,fellow eye group and normal eye group (C1 q:F=8.810,P =0.003;C3:F =14.490,P＜0.001),and expression levels of C1q and C3 proteins in the defocused eye group were significant higher than those of the fellow eye group (C1q:q=4.14,P=0.01 ;C3:q=4.71,P=0.005) ;while no significant differences were found between the fellow eye group and the normal eye group (C1q:q =1.61,P =0.27; C3:q =2.82,P =0.070).Conclusions The expression levels of C1q and C3 up-regulate in the retinas with lens-defocused myopic animal model.Excessive complement activation in retinas may be involved in the development of myopia.%背景 高度近视严重危害视功能,探究其发病机制有助于疾病的预防和治疗,是眼科领域的重要研究方向之一.补体系统参与病理性近视的发生和发展,但近视眼视网膜中补体成分的变化研究较少.目的 观察负透镜诱导豚鼠离焦性近视发生时视网膜组织中补体C1q和C3表达的变化. 方法 将12只出生3d的有色豚鼠按随机数字表法分为正常对照组和透镜诱导组,透镜诱导组豚鼠的左眼用-10D聚甲基丙烯酸甲酯(PMMA)镜片缝合在眼周皮肤4周以诱导近视眼模型,作为透镜诱导眼,右眼用平光PMMA以同样的方法进行缝合作为对侧对照眼,正常对照组的右眼作为正常对照眼.诱导后4周测量各组豚鼠的屈光度,过量麻醉法处死豚鼠并分离视网膜,采用Western blot法检测各组豚鼠视网膜中C1q和C3蛋白的表达.结果 实验后4周,透镜诱导眼、正常对照眼和对侧对照眼平均等效球镜屈光度(SE)分别为(-1.21±0.71)、(+2.46±0.75)和(+1.75±0.50)D,3个组间比较差异有统计学意义(F=51.55,P=0.69),正常对照眼与对侧对照组间豚鼠的平均SE差异无统计学意义(q=2.62,P=0.08),而透镜诱导眼豚鼠的平均SE明显高于对侧对照眼,差异有统计学意义(q=10.92,P＜0.01);透镜诱导眼、对侧对照眼和正常对照眼视网膜组织中C1q和C3蛋白的表达量明显不同,差异均有统计学意义(C1q:F=8.810,P=0.003;C3:F=14.490,P＜0.001),其中透镜诱导眼视网膜中C3和C1q蛋白表达明显高于对侧对照眼,差异均有统计学意义(C1q:q=4.14,P=0.01;C3:q=4.71,P=0.005),而对侧对照眼视网膜中C1q和C3蛋白表达与正常对照眼间差异均无统计学意义(C1q:q=1.61,P=0.27;C3:q=2.82,P=0.07).结论 离焦性近视发生过程中存在视网膜组织的补体活化状态,补体过度活化可能参与近视的发生.
展开▼
