山东省某些高危人群HIV-1感染者分子流行病学研究

摘要

目的　了解流行于山东境内HIV-1毒株的亚型分布及变异情况，分析其来源并推测其流行趋势。方法　采集了25份HIV-1抗体阳性感染者的全血分离单核细胞(PBMC)，提取前病毒DNA，经nested-PCR扩增HIV-1膜蛋白(env)基因的C2-V3区并进行序列测定和亚型分析。结果　25份HIV-1阳性感染者的PBMC样品中扩增到24份可用于序列测定的HIV-1env基因片段，经序列测定和基因分析鉴别出共有5种HIV-1M亚群基因亚型：A、B、C、E和B＇亚型。13名有偿献血人员均为B＇(泰国B)亚型，10名回国劳工及1名回国劳工的女性配偶中存在A、B、C、E四种亚型。结论　山东省HIV-1各种亚型毒株感染情况复杂，因此应加强对献血者及回国劳工等高危人群的检测和控制，以防HIV-1各亚型毒株在全省的蔓延。%Objective The aim of this study was to understand the distribution of HIV - 1 strain sub-types and their variation in Shandong Province and to analyze their source and predict their epidemic trend. Methodrn We collected the whole blood from 25 anti - HIV- 1 antibody positive cases and separated the peripheral bloodmononuclear cells. The provirus DNA was extracted and amplified by nested- PCR, after which the HIV - 1 mem-brane protein gene(env) C2 - V3 region was sequenced and its subtype was analyzed. Results Among 25 HIV -1 antibody positive cases,24 HIV- 1 env gene fragments were amplified and analyzed.5 subtypes of Group HIV-1 M were identified among these cases,that are subtypes A,B,B' ,C and E.13 occupational blood donors were allclassified as subtype B' (Thailand B').A,B,C or E subtypes were discovered in 10 returned laborers workingabroad and 1 female spouse of one laborer. Conclusion The condition of HIV - 1 subtype infection was compli-cared in Shandong Province. So, it is important to strengthen the surviellance and control of HIV - 1 subtype straininfection in high risk populations especially the occupational blood donors and returned laborers working abroad toprevent its spread in Sbandong Province.