密码子优化提高重组腺病毒中人轮状病毒G1VP7和G3VP7基因的表达量

摘要

Objective To increase the recombinant adenovirus vector mediated human rotavirus G1VP7 and G3VP7 gene expression through coden optimization. Methods We have artificially synthesized two genes of group A human rotavirus that encode G1VP7 and G3VP7 according to the human biased codan. The modified genes were transfected into 293 cells using adenovirus vectors and the gene products,the respective proteins were produced. The expression level of optimized genes and wild type genes was detected by Western Blot. Results A remarkable increase of the expression level of optimized G1VP7 and G3VP7 genes in comparison with the wild type control. Conclusion The coden optimization indeed help increasing the recombinant adenovirns mediated human rotavirus gene expression,which indicated the potential application of such recombinant adenovirases in the development of adenoviral-vectored rotavirus vaccines.%目的 通过密码子优化提高重组腺病毒中人轮状病毒G1VP7和G3VP7基因的表达量.方法 人工合成按照人的优势密码子优化的人轮状病毒G1VP7和G3VP7基因,包装重组腺病毒后,与未优化的相同基因的重组腺病毒用Western Blot方法比较其表达量.结果 密码子优化的人轮状病毒G1VP7和G3VP7基因,与未优化的相同基因的重组腺病毒比较,其蛋白表达量大大提高.结论 密码子优化确实提高了重组腺病毒中人轮状病毒G1VP7和G3VP7基因的表达量,可望满足腺病毒载体疫苗的研发需要.