北京市男男性接触HIV-1感染者膜蛋白基因序列测定及亚型分析

摘要

Objective To comprehend the latest HIV-1 epidemic tendency and the character of V3 loop in MSM population of Beijing. Methods The C2-V3 regions of the HIV envelop gene were amplified by nest-PCR and sequenced from 11 HIV-1-infected MSM in Beijing in 2007. The subtype and sequences of V3 loop was analyzed. Results There are 4 subtype B strains, 5 CRF AE, 1 CRFOTBC and 1 CRFI5-01B strains within all 11 strains. There are five types of central motifs of the 11 samples, in which GPGR and GPGQ are most common. Conclusion Recombination subtype of HIV-1 are spread extensively in MSM population of Beijing.%目的 了解北京市男男性接触人群(MSM)中HIV-1的最新流行趋势及膜蛋白V3环序列特征.方法 巢式聚合酶链式反应(n-PER)扩增2007年提取的北京市男男性接触HIV感染者基因组DNA样品,对膜蛋白基因C2-V3区测序,进行病毒亚型及V3环序列特点分析.结果 11例样本中,4例是欧美B亚型,5例是AE重组亚型,1例是BC重组亚型,1例是01B重组亚型.V3环顶端四肽以GPGQ和GPGR为主.结论 北京市男男性接触HIV-1感染者中重组亚型呈蔓延流行趋势.