Objective To explore the relationship of infection of Ureaplasma urealyticum(UU),HPV loading dose and CIN or cervical cancer, also the relationship of infection of Ureaplasma urealyticum,HPV genotype and CIN or cervical cancer. Methods One hundred and twenty-four patients in The sixth and second Affiliated Hospital of Sun Yat-sen University with CIN or cervical cancer were selected to be case group from September 2005 to January 2009. Three hundred health examining women were selected randomly by stratified sampling from medical examination center of the hospital during synchronization as control group. We detected Ureaplasma urealyticum, DNA quantitative expression of cervical secretions from patients with CIN or cervical cancer by means of Fluorescent quantitative PCR(FQ-PCR) and also HPV DNA by both Hybrid capture Ⅱ and flow-through hybridization and gene chip. Results The positive rate of UU in case group was 68. 5% , in control group was 38.7%. Compared positive rate of UU between case group and control group, the difference had statistical significance. In 124 cases detected for both UU DNA and HPV DNA, 58.9% were positive for both UU DNA and HPV DNA. Conclusion There may be some relationship between UU positive of lower genital tract and cervical intra-epithelial neoplasia or cervical cancer, especially there may be some relationship between UU positive and high-grade lesion of cervix.There maybe interaction between UU and infection of HPV.%目的 了解宫颈上皮内瘤变(CIN)宫颈癌患者下生殖道解脲脲原体感染情况,初步探讨解脲脲原体(UU)、HPV与CIN/宫颈癌的相关性.方法 选取2005年9月至2009年2月中山大学附属第六医院及第二医院因宫颈上皮内瘤变或宫颈鳞癌住院的患者124例为病例组.随即抽取同期医院体检中心健康体检女性300例为对照组.采用荧光定量PCR方法进行宫颈分泌物解脲脲原体DNA检测.用杂交捕获Ⅱ代和导流杂交基因芯片技术检测HPV感染情况.结果 病例组解脲脲原体阳性率68.5%,对照组解脲脲原体阳性率38.7%.病例组和对照组之间UU阳性率比较,差异有统计学意义.124例行HPV检测病例中,UU和HPV均阳性者占58.9%.结论 女性下生殖道解脲脲原体感染与宫颈上皮内瘤变/宫颈癌有一定的相关性.解脲脲原体感染与宫颈病变程度相关,尤其与高度宫颈上皮内病变和宫颈癌相关.UU对HPV感染导致的CIN/宫颈癌可能存在协同作用.
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