目的 研究手足口病(HFMD)患者外周血淋巴细胞CD95、HLA-DR活化分子表达的变化及其意义.方法 以健康儿童作为对照,采用流式细胞术,检测58例手足口病患者外周血淋巴细胞CD95标志、T细胞亚群及其HLA-DR抗原表达.结果 HFMD患者外周血CD3~+T细胞的百分率(63.82±7.74)%与健康对照组比较差异有统计学意义(P<0.001),CIM~+T细胞的百分率(34.29±7.33)%明显低于正常对照组(P<0.005);HLA-DR~+淋巴细胞(28.30±7.61)%比正常对照组显著增高(P<0.005);CD8~+T细胞表达HLA-DR水平(1.34±1.12)%明显高于对照组(P<0.005);淋巴细胞CD95表达差异无统计学意义(P>0.05).结论 HFMD患者存在细胞免疫功能紊乱和淋巴细胞异常激活现象,T淋巴细胞的激活以CD8~+T细胞为主,其在抗病毒感染中起着重要作用.%Objective To investigate the expression of CD95 and special marker for activation of peripheral blood lymphocytes in patients with hand foot and mouth disease (HFMD) and its significance. Methods Immunofluorescent two-color flow cytometry was used to study the expression of CD95 and HLA-DR on lymphocytes in 58 patients with HFMD and 34 normal controls. Results Expression of CD3~+ T cells was significantly lower in patients (63.82±7.74)% than that in controls (P<0.001), meanwhile the expression of CD4~+ T cells was (34.29±7.33 ) %, significantly lower than that of the controls (P<0.005). The percentage of lymphocytes expressing HLA-DR in patients was(23.77±5.78)%, significantly higher than that of the controls (P<0.005 ). Significant difference was observed in the expression of HLA- DR on CD8~+ T cells in patients(1.34±1.12)% as compared with controls (P<0.005). No significant difference in the expression of CD95 on lympnocytes was observed between patients and the controls (P>0.05). Conclusion The findings support that cellular immunodeficiency exists in patients and that lymphocytes were abnormally activated in the patients. The activation of peripheral blood T lymphocytes in patients mainly involves CD8~+ subset and it may play an important role in the immune response to antiviral infection.
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